Haploid production can reduce the time required to produce improved varieties of a particular plant, increase yield, and improve quality. Haploid production techniques are also combined with other available biotechnology tools such as marker-assisted selection (MAS), induced mutagenesis, and genetic engineering techniques to accelerate crop breeding. The use of chromosome doubling (spontaneous or induced) can speed up the breeding process by obtaining completely pure plants with unique genetic recombination in a single generation. Different methods of haploid induction have been developed, including solanaceous reproduction, interspecific hybridization, radiation pollination of pollen, anther or microspore cultures for in vitro androgens, etc. Double haploid is a genotype formed when haploid cells successfully undergo spontaneous or artificially induced chromosome doubling. So far, the double haploid technique has been used in the breeding of autogamous varieties or in the breeding of autogamous lines for further use in the production of hybrids of heterozygous varieties.
Fig. 1. Number of generations to reach genetic purity (homozygosity) through: (A) conventional inbreeding; (B) doubled haploid technology. (Prasanna et al., 2012)
Our haploid identification solution
Our agricultural genome sequencing services provide abundant genomic resources for most crops, high-throughput cost-effective phenotypes, genotyping tools, and information on markers and genomic regions associated with agronomically beneficial traits. These sequencing information provides support for double haploid breeding solutions of various crops. This approach allows for the production of completely pure lines in a matter of months and significantly reduces the time required to establish new varieties. This technology also allows breeders to make experimental crosses with the progeny of lines rather than inbred lines.
Application of double haploid technology in crop breeding
Double haploid technology has been used in plant breeding and genomics research. CD Genomics is committed to developing double haploids, genomic technologies, and integrated data management to accelerate the development of crop varieties with specific attributes.
QTL Mapping: Double haploids are valuable for QTL mapping, a key process for identifying genetic markers associated with desired traits. CD Genomics provides double haploids identification service to facilitate QTL studies, enabling breeders to pinpoint genomic regions responsible for specific traits.
Marker development: Double haploids are an excellent resource for marker development. By analyzing genomic variation between haploids and double haploids, breeders can identify markers associated with desired traits. CD Genomics offers comprehensive high-throughput sequencing technology to develop markers.
Transformation: Double haploids are ideal candidates for genetic transformation. Our sequencing services facilitate the transformation of double haploid plants to introduce or modify specific genes to develop improved crop varieties with enhanced traits.
Our strengths and features
Cutting-edge technology. We use the latest genomics technologies to ensure accurate and efficient haploid identification.
Comprehensive services. We provide end-to-end solutions from haploid induction to advanced applications under one roof.
Flexibility. We offer in vivo and in vitro haploid identification technologies to meet a wide range of research and breeding requirements.
Customization. Our services are tailored to meet the specific needs of our clients, ensuring that their targeted efficiencies are achieved precisely and efficiently.
As a leading provider of agricultural genomics services, CD Genomics is committed to providing whole genome sequencing to characterize double haploids. We provide genome sequencing information to help our customers establish a new, simple, and efficient method for breeding diploids in crops. If you are interested, please feel free to contact us.
Reference
Prasanna, B. M., Vijay Chaikam, and George Mahuku. Doubled haploid technology in maize breeding: theory and practice. Cimmyt, 2012.
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CD Genomics is propelling the future of agriculture by employing cutting-edge sequencing and genotyping technologies to predict and enhance multiple complex polygenic traits within breeding populations.
Fig. 1. Number of generations to reach genetic purity (homozygosity) through: (A) conventional inbreeding; (B) doubled haploid technology. (Prasanna et al., 2012)
