Ligase Detection Reaction (LDR), which enables the identification of genetic polymorphic loci by using high temperature ligases, is a common method for detecting SNPs. In the presence of Taq DNA ligase, the ligation reaction can only occur when the left and right oligonucleotide probes are fully complementary to the target DNA sequence and there is no gap between the two probes, otherwise the ligation reaction cannot proceed, and finally the fragment length is scanned by capillary electrophoresis to achieve the identification of gene polymorphic loci, which is a common method for detecting SNPs.

CD Genomics provides LDR services, through improved LDR technology, our services can do multiple SNPs at once, while the success rate and accuracy of our data, has been greatly improved.

Technical features

• High success rate of SNP design
• High accuracy of data results
• Lower experimental cost, higher experimental output
• High throughput, high sensitivity, good reproducibility
• High specificity, good generality and wide range of application

Our services can be applied to the following research areas

• Plant and animal trait correlation analysis
• Plant and animal marker-assisted selection
• Detection of genetic variation in plants and animals
• Plant virus detection
• SNP genetic identification
• Environmental monitoring
• Biosafety

Instructions for providing samples

The client will need to provide information on the SNP site , in the case of species without rs numbers, such as cattle, chicken, fish, the exact sequence 200 bp upstream and downstream of the SNP site, the type of mutation at the SNP site, and the presence of other sites within 25 bp upstream and downstream of the site.

Service workflow

Fig 1. LDR service workflow

Our advantages and features

• Professional and personalised subject design guidance, including assistance with gene selection, SNP selection strategies, and guidance on data analysis.
• Multiple quality controls to ensure data reliability and accuracy.
• Autonomous synthesis of universal primers and specific probes to shorten time and improve efficiency.
• Self-developed probe synthesis solution with high sensitivity and accurate detection results.
• International advanced instruments with multiple typing platforms to meet the needs of different projects of our customers.

CD Genomics has a professional technical team with a wealth of experience in SNP typing, which has been unanimously approved by our clients and is a technological leader in its field at home and abroad. We have been able to type all types of SNPs in the genome, and in addition, we can achieve a success rate of over 95% for specimens that pass quality control. So far, we have successfully completed SNP testing for a large number of species through LDR. If you are interested in us, please feel free to contact us.