Genotyping by sequencing (GBS), as a method to simplify genome sequencing, is used to screen for single nucleotide polymorphisms (SNPs) in a variety of plant and animal species and populations, using phenotype-related SNPs to aid genomic selection and improvement of plant and animal species, and has important applications in the genomics-assisted breeding process. The addition of high density SNP markers to genetic maps or breeding populations through GBS is valuable in advancing genetic breeding research in plants and animals.

CD Geomics offers GBS to help researchers conduct cost-effective, high-throughput genotyping studies for larger plant and animal populations, advancing the breeding of agricultural plants and animals, saving time and costs in the improvement of agricultural plant and animal varieties, and providing GBS services to help breeders select genomes for new germplasm or species, determine genomic or diversity in population structure species, and help researchers better understand the relationship between genetic factors and phenotype production in plants and animals.

Features of GBS

• Simpler, faster, more specific and highly reproducible for large genomic species.
• Focused on thousands of markers, providing a customised, flexible and scalable cost-effective, high-throughput genotyping solution.
• Sequencing is lower cost, with simplified steps and more efficient turnaround to reach important regions of the genome.

GBS in plant and animal research

• Marker-assisted selection and breeding
• Genetic mapping
• Population genetic analysis
• Predicting phenotypes and selecting improved varieties
• Parentage and traceability

GBS workflow

Fig 1. GBS workflow

Our advantages and features

• Experienced. Our experts and technical team have a solid theoretical foundation and a wealth of practical experience.
• Technical support. We offer a full technical support service and our experts will discuss with you if you need any help with your project.
• Complete and attentive after-sales service. We reduce your worries about the progress of your project.
• Personalised experimental protocols. We have more than a dozen restriction endonucleases such as HaeIII, EcoRI, NlaIII. We can select the most suitable restriction endonuclease for you according to your project needs and characteristics.
• Original imported reagents. The sequencing quality is guaranteed to be above 85% Q30, the efficiency of enzyme capture is above 95%, and the captured enzyme segments are well distributed on the chromosome.
• Advanced sequencing platform. Higher coverage of the genome and greater volume of captured data.

CD Genomics provides comprehensive target genotyping services, and we have extensive experience in sequencing services for targeted genotyping of plants and animals. To date, we have successfully provided genotyping services for dozens of species such as maize, barley, rice and fish, providing additional genetic data for hybrid selection of these species through analysis of their genetic structure and their hybrid offspring. If you are interested in us, please feel free to contact us.