At CD Genomics, our SELECT-m6A Sequencing service offers precise, single-base resolution mapping of m6A modifications, unlocking critical insights into RNA regulation. Whether you're studying gene expression, RNA stability, or biomarker exploration, we provide a streamlined, cost-effective solution for m6A analysis.
Pinpoint Accuracy: Get high-resolution, single-base precision results.
No Antibodies: Avoid the variability and costs of antibody-based methods.
Customizable Analysis: Tailored to your specific research needs, from single genes to broad profiles.
Sensitive & Cost-Effective: Ideal for studies with limited RNA samples.
m6A (N6-methyladenosine) is a pivotal RNA modification that plays a significant role in regulating gene expression and cellular processes. Here's why it's so crucial:
Gene Regulation: m6A acts as a "molecular switch" that turns genes on or off, influencing their expression.
RNA Processing: It affects RNA stability, splicing, translation, and degradation.
Cellular Function: m6A regulates cellular responses to stress, development, and progression in diseases like cancer and neurodegenerative disorders.
For researchers, this means:
Uncover Mechanisms: Understanding m6A gives insight into how RNA orchestrates cellular behavior.
Identify Biomarkers: m6A modifications can pinpoint disease biomarkers.
Therapeutic Potential: Studying m6A offers the potential to develop new treatments for various conditions.
In short, m6A is a central factor in gene regulation and cellular health, holding tremendous potential for both research and therapeutic advancements.
Why Choose SELECT-m6A Sequencing? A Comparison with Other Technologies
When choosing the right method for m6A modification detection, multiple options exist. However, SELECT-m6A Sequencing stands out due to its reliability and efficiency, offering key advantages over other commonly used techniques. Here's how it compares:
At CD Genomics, we offer an efficient and precise SELECT-m6A Sequencing service that empowers researchers to explore m6A RNA modifications in detail. With this technology, every subtle m6A modification is captured with single-base resolution and high sensitivity, ensuring the most reliable results for your research.
How SELECT Works: The Process
RNA Extraction
We begin by extracting high-quality RNA from your samples. This method is particularly effective for working with limited RNA samples, ensuring reliable results even with minimal material. Unlike conventional techniques, SELECT delivers consistent outcomes from small RNA inputs.
m6A Enrichment
We utilize an enzymatic enrichment approach to selectively isolate RNA fragments with m6A modifications. This approach eliminates the complexity and costs of traditional antibody-based methods, providing more accurate and uncontaminated data.
Amplification and Sequencing
After enrichment, the RNA fragments are amplified and sequenced using high-resolution technology. SELECT achieves single-base resolution, allowing for precise identification of each m6A modification, with an error rate kept below 5%.
Data Analysis
Once sequencing is complete, we process the data using advanced bioinformatics tools. This results in detailed maps of m6A modification sites across your RNA samples. We present the data in a clear, visual format to help you easily interpret the results and gain meaningful insights.
Why Choose Our SELECT-m6A Sequencing?
Single-Base Resolution, Precise Detection
With single-base resolution, SELECT pinpoints the exact location of each m6A modification, providing unmatched precision for your research.
No Antibodies, Simplified Process
Unlike traditional methods that rely on antibodies for enrichment, SELECT uses enzymatic enrichment. This reduces variability, eliminates extra costs, and ensures more stable, reproducible results.
Minimal RNA Input
SELECT requires just 1 μg of RNA, making it perfect for studies with limited RNA samples. You can maximize the use of precious material without compromising data quality.
High Sensitivity for Low-Abundance Modifications
SELECT can detect low-abundance m6A modifications, identifying subtle changes that may be critical in understanding disease mechanisms or gene regulation.
Comprehensive Bioinformatics Support
Beyond sequencing, our bioinformatics team helps you interpret the data through in-depth analysis. From mapping m6A sites to comparing abundance differences, we ensure you gain valuable insights for your research.
Cost-Effective and Streamlined
By eliminating antibody-based enrichment, SELECT reduces both costs and complexity. This streamlined process not only makes your research more affordable but also saves valuable time.
Workflow
Client-Centric Approach: Every Step of the Way
Bioinformatics
Bioinformatics Analysis Tailored for SELECT-m6A Sequencing Data
Generating high-quality sequencing data is only part of the process. Transforming raw reads into meaningful biological insights requires sophisticated bioinformatics support. At CD Genomics, we ensure that your SELECT-m6A Sequencing results are thoroughly analyzed to meet your specific research goals.
Comprehensive Data Processing
After sequencing, our pipeline cleans and filters raw data to accurately identify m6A modifications, aligning reads to the reference transcriptome and confirming modification sites at single-base resolution.
Intuitive Mapping and Visualization
To make complex data more accessible, we provide graphical outputs such as heatmaps, genome browser tracks, and site-specific coverage plots, helping you quickly identify regions enriched with m6A marks.
Differential Modification Analysis
Our bioinformatics workflow compares m6A patterns between different sample groups or conditions, revealing how m6A modifications change in response to treatment, stress, or disease, providing deeper insights into RNA regulation dynamics.
Functional Annotation and Interpretation
We annotate detected m6A sites with gene function and pathway information, placing modifications in the context of broader biological processes. This supports hypothesis generation and experimental planning.
Customised Reporting
Each project receives a comprehensive report summarizing key findings, with publication-ready figures and clear data interpretation. We make it easy to incorporate your results into manuscripts or presentations.
Applications
Applications of SELECT-m6A Sequencing for RNA Research
Mapping RNA Methylation Landscapes
SELECT-m6A sequencing achieves single-base resolution mapping of N6-methyladenosine (m6A) modifications. This precision enables:
Identification of methylation sites within coding regions, untranslated regions (UTRs), and non-coding RNAs
Correlation of modification patterns with RNA secondary structures (e.g., G-quadruplex regions)
Quantitative analysis of methylation stoichiometry across transcript isoforms
Investigating RNA Stability Mechanisms
The technology detects dynamic m6A changes under cellular perturbations:
