hMeDIP-seq
Hydroxymethylated DNA Immunoprecipitation Sequencing (hMeDIP-Seq) is a genome-wide detection of hydroxymethylated regions through 5hmC-specific antibody enrichment combined with high-throughput sequencing technology. It can be widely used in the study of the relationship between hydroxymethylation and diseases and the study of hydroxymethylation in embryonic development.
Specific hMeDIP-seq Approaches We Offer
Service Introductions
- CD Genomics offers services to map 5-hmC in the genome using 5-hmC DNA immunoprecipitation (hMeDIP).
- Our assays include immunoprecipitation of genomic DNA using our monoclonal 5-hmC antibody.
- We sequence the enriched DNA using Illumina Next-gen sequencing technology to generate a whole genome map of 5-hmC methylation.
Analysis Contents
| hMeDIP-seq | Analysis Contents |
| Basic Analysis | 1. Raw data removal of low quality, removal of joint contamination processing 2. quality assessment after data processing 3. sequencing data genome alignment 4. genome sequencing depth and coverage analysis 5. detection of hydroxymethylated enriched regions (peaks) and basic statistics 6. distribution of genomic components in hydroxymethylated enriched regions (peaks) 7. visualization of hydroxymethylation-enriched regions 8. distribution characteristics of hydroxymethylation levels on different genomic elements 9. distribution of hydroxymethylation levels on different gene structural elements 10. detection of differentially hydroxymethylated enriched regions (DhMRs) 11. location of differentially hydroxymethylated enriched regions (DhMRs) and annotation of related genes 12. GO enrichment analysis of genes associated with differentially hydroxymethylated enriched regions (DhMRs) 13. KEGG enrichment analysis of genes related to differential hydroxymethylation enrichment regions (DhMRs) |
| Senior Analysis | 14. Inter-sample genome-scale hydroxymethylation level clustering analysis 15. inter-sample genome-scale hydroxymethylation level principal component analysis (PCA) 16. disease-related hydroxymethylation analysis and functional prediction |
Technical route
Genomic DNA - DNA fragmentation - hMeDIP enrichment - library construction - PE150 sequencing - normalization analysis - advanced analysis
Services Advantages
- hMeDIP-seq covers the entire genome of CpG and non-CpG 5mC.
- hMeDIP-seq covers 5mC in dense, low-density and repetitive regions.
- hMeDIP-seq is based on antibody selection independent of sequence and does not enrich for 5hmC due to antibody specificity.
Workflow
Our Capabilities
- Specific detection of hydroxymethylation: 5-hmC antibody is able to distinguish between methylation and hydroxymethylation signals and specifically detect hydroxymethylation regions.
- High flexibility: Capable of directly sequencing highly hydroxymethylated fragments of any species without the need for known genomic sequence information.
- Wide detection range: covers the entire genome-wide hydroxymethylated region.
- High precision: Capable of localizing within 50 bases of the actual binding site.
- Digital signal: direct sequencing and quantification of hydroxymethylated fragments without the cross-reactivity and background noise associated with the fluorescent analog signal of traditional microarray hybridization.
Sample Requirements
- Customers need to submit purified DNA, frozen tissue or cell precipitate.
- Genomic DNA: ≥3ug
- Sample concentration >50ng/ul; no RNA and protein contamination
Service Process
Deliverables
1. Related experimental results raw data 2. Experimental report 3. Data analysis 4. Image and result analysis 5. Bioinformatics analysis results 6. Details in hMeDIP-seq for your writingOur Features
- CD Genomics will complete your project on time and efficiently. We have professional after-sales service.
- CD Genomics works with scientists from many biotechnology companies. We have extensive knowledge and experience to provide quality assurance services.
Why Choose Us?
CD Genomics is a professional hMeDIP-seq technology provider, supporting a variety of sequencing services. We are able to perform individual tests at the most precise level, as well as design and complete a suite of services as a solution to the subject project. CD Genomics has accumulated years of quintessence in the field of hMeDIP-seq, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects. If you would like to know more about this service, please feel free to contact us.