O8G-Seq
Reactive oxygen species (ROS) and free radicals play major roles in normal biological functions. However, they can also react rapidly with biomacromolecules, such as nucleic acids, proteins, or lipids, and alter their biological functions. Reactive oxygen species formed within the cell can produce 8-oxo-7,8-dihydroguanine (O8G) in mRNA, which can cause base mispairing during gene expression. In order to systematically reveal the oxidative modification of RNA O8G, CD Genomics provides mature MeRIP Seq technical services for RNA O8G oxidation.
Figure 1. A schematic representation of the abnormal protein synthesis induced by O8G incorporation into mRNA (Dai,2018)
Specific O8G-Seq Approaches We Offer
Methods
- RNA fragments were randomly interrupted
- The RNA O8G oxidation specific antibody was co incubated with RNA fragments
- Enriched O8G oxidation modified fragments were sequenced.
- At the same time, it is required to sequence an input sample in parallel, and the control sample is an RNA fragment without IP reaction.
Our O8G-Seq are available for a wide range of sequencing applications:
- Complete transcriptome sequencing of O8G
- Sequencing of O8G cyclic RNA
- O8G LnRNA sequencing
- O8G mRNA sequencing
Our bioinformatics analysis services include, but are not limited to:
- Sequencing data quality control
- Comparison with reference genome
- Identified oxidation sites
- Length distribution of oxidation sites and motif analysis results
- Differential oxidation sites
- GO and Pathway analysis results of differential oxidation sites
Service Advantages
- Specific enrichment and detection of RNA fragments oxidized by O8G
- RNA O8G oxidation detection in the whole transcriptome
- Targeted detection of O8G oxidation of different types of RNA molecules
- It can detect the O8G oxidation of almost any eukaryote
Workflow
Our Capabilities
- Wide detection range: Whole-transcriptome wide RNA O8G oxidation profiling.
- Flexible sample requirements: Cells, tissues or RNA are acceptable
- Specialized bioinformatics analysis: Strong bioinformatic team, professional O8G oxidation data analysis.
Sample Requirements
- Cell samples
The number of cells shall not be less than 1×108.
- Fresh tissue samples
The quantity of the tissue samples should be between 500 mg - 5g.
- RNA samples
The quantity of RNA sample should be no less than 30 μg, RNA purity: OD260/280 = 1.8~2.2; OD260/230 ≥ 1.5; RNA quality: 28S:18S ≥ 1.5, RIN ≥ 7.
Service Process
Deliverables
- Related experimental results raw data
- Experimental report
- Data analysis
- Image and result analysis
- Bioinformatics analysis results
- Details in O8G -Seq for your writing
Our Features
- CD Genomics will complete your project on time and efficiently. We have professional after-sales service.
- CD Genomics works with scientists from many biotechnology companies. We have extensive knowledge and experience to provide quality assurance services.
Why Choose Us?
CD Genomics is a company that provides professional and comprehensive O8G -Seq services. We have years of experience to meet your specific project needs in using epigenomics research to add value to your research projects. CD Genomics can provide you with personalized solutions to help you thrive every step of the way around your interest in your workflow. If you would like to know more about this service, please feel free to contact us.
Reference
- Dai, Da-Peng, et al. Transcriptional mutagenesis mediated by 8-oxoG induces translational errors in mammalian cells. Proceedings of the National Academy of Sciences 115.16 (2018): 4218-4222.