Sample Guidelines

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Shipping Guidelines

Use FedEx, DHL, or UPS for shipments and ensure samples arrive on weekdays.
Notify us via email with the Sample Submission Form and tracking number before shipping.
Package securely:
- Use 1.5 mL tubes sealed with parafilm in rigid containers with cushioning.
- For large projects, use sealed PCR plates or strip tubes with frozen blue ice/dry ice.
Label tubes with a black permanent marker on the lid and side.
DNA: Ship in 70% ethanol at room temperature or with ice packs if in water/TE buffer.
RNA, cells, bacteria, and frozen tissue: Freeze with liquid nitrogen and ship on dry ice.
Blood: Use anticoagulant tubes, wrap in bubble wrap, and place in rigid boxes.

SAMPLE SUBMISSION GUIDELINES

Epigenome Epitranscriptome Single Cell Methylation Sequencing

SUGGESTIONS OF SAMPLING

SAMPLE SUBMISSION GUIDELINES

DNA Methylation Sequencing

Submission Medium: DNA should be submitted in DNase-free water, Elution Buffer, or 10 mM Tris pH 8.0.
Quality Standards: Target an OD260/280 ratio of 1.8–2.0. All DNA must be RNase-treated and free from degradation or contamination.
Shipping: Ship with ice packs.
Amount and Measurement: The required DNA amount varies by application; consult the Sample Requirements table. Use fluorometric methods for concentration measurement, or double the values if using spectrophotometry.

Service	Sample Type	Recommended Quantity	Minimum Quantity	Minimum Concentration
DNA Methylation Microarray	DNA	≥ 1 µg	500 ng	8 ng/µL
MeDIP-Seq/hMeDIP-seq	Genomic DNA	≥ 2 μg	1 μg	20 ng/μL
WGBS (Whole Genome Bisulfite Sequencing)	Genomic DNA	≥ 1 μg	200 ng	10 ng/μL
	Cell	≥ 1 x10⁶
	Tissue	≥ 50 mg
RRBS (Reduced representation bisulfite sequencing)	Genomic DNA	≥ 1 μg	20 ng	20 ng/μL
	Cell	≥ 5 x10⁶	3×10³
	Tissue	≥ 30 mg
Targeted Bisulfite Sequencing	Genomic DNA	≥ 500 ng	50 ng	10 ng/μL
	Cell	≥ 1×10⁶
	Tissue	≥ 20 mg
oxWGBS-Seq	Genomic DNA	≥ 3 µg	1 µg	30 ng/μL
oxRRBS-Seq	Genomic DNA	≥ 2 µg		50 ng/μL
oxTBS-Seq	Genomic DNA	≥ 1 µg		20 ng/μL
DNA 6mA-IP-Seq	Genomic DNA	≥ 5 μg		20 ng/μL
Epityper	Genomic DNA	≥ 1 µg
ChIP-seq	ChIPed DNA	≥ 10 ng	5 ng	1 ng/µL
	Cell	≥ 2 x10⁷	1 x10⁵
	Tissue	≥ 500 mg
DAP-seq	TF	≥ 5 µg	1 µg	20 ng/µL
	Cell	≥ 5 x10⁶
	Tissue	≥ 500 mg	200 mg
ATAC-seq	Cell	≥ 1 x10⁶	5 x10⁴
ATAC-seq	Tissue	≥ 500 mg	200 mg
CUT&Tag	Cell	≥ 1 x10⁶	5 x10⁴
CUT&Tag	Tissue	≥ 500 mg	200 mg

RNA Methylation Sequencing

Submission Medium: RNA can be submitted in RNase-free water, RNA Stabilization Reagent, or 10 mM Tris pH 8.0.
Quality Standards: Samples must be DNA-free with the following ratios:
OD A260/A280: ≥ 1.8
OD A260/A230: ≥ 1.8
RIN (RNA Integrity Number): ≥ 6
Shipping: Use dry ice for shipping.
Amount Required: The total RNA amount varies by application; refer to the Sample Requirements table for guidance.
Concentration Measurement: Use fluorometric methods for concentrations; if using spectrophotometry, double the values.

Service	Sample Type	Recommended Quantity	Minimum Quantity	Minimum Concentration
RIP-seq	IPed RNA	≥ 100 ng	40 ng	5 ng/µL
	Cell	5×10⁷
	Tissue	500 mg	200 mg
eCLIP-Seq	IPed RNA	≥ 100 ng	40 ng	5 ng/µL
	Cell	≥ 3×10⁷
	Tissue	≥ 500 mg	200 mg
MeRIP (Methylated RNA Immunoprecipitation)	Total RNA	≥ 10 μg	2 μg	1 ng/µL
	Cell	≥ 1×10⁷
	Tissue	≥ 500 mg	200 mg
RNA BS-seq (RNA Bisulfite Sequencing)	Total RNA	≥ 10 μg
	Cell	≥ 1×10⁷
	Tissue	≥ 500 mg	100 mg

Single Cell Methylation Sequencing

Service	Sample Type	Recommended Quantity	Minimum Quantity
scATAC-seq	Single cell nuclei suspension	1×10⁶, Nuclei < 40 μm, complete nuclear membrane, and the proportion of nuclei > 95%	5×10⁵
	Single cell suspension	≥1×10⁶ Cell Viability>80%, Cell concentration 700~1200 cells/μL,	5×10⁵
	Fresh tissue	200 mg
	Whole blood anticoagulated with EDTA	≥ 5mL
ScWGBS	Cell lines, primary cells, fresh tissue, frozen cells	Use 200μl PCR tubes to store cells (single or multiple cells), 5μl of lysate per tube, and no more than 1μl of buffer when collecting cells. ≥3 biological replicates
ScRRBS	Cell lines, primary cells, fresh tissue, frozen cells	Use 200μl PCR tubes to store cells (single or multiple cells), 5μl of lysate per tube, and no more than 1μl of buffer when collecting cells. ≥3 biological replicates

Suggestions of Sampling

It is well-established that the integrity of input nucleic acids is directly correlated with the quality of sequencing data produced. To address this crucial aspect of genomic research, CD Genomics provides an extensive array of sample extraction services tailored to diverse sample types. Our team is readily available for consultation, offering bespoke solutions that cater to the specific requirements of each sample type.

Sample Type	Quantity Recommended	Shipping Method
Cell	1x10⁶ cells	Dry ice
Fresh Frozen Tissue	10 mg	Dry ice
FFPE	4 FFPE slides, thickness 5-20 um, area >150 mm²	Room temperature/Blue ice
Viral Particles	5 x10⁹	Dry ice
Stool	100 mg	Dry ice
Swabs	2 tubes/sample, 1 swab/tube	Room temperature
Saliva	1 mL	Dry ice/Blue ice
Soil	100 mg	Room temperature/Blue ice
Water	50 mL	Room temperature/Blue ice
Plasma/Serum	10 mL	Dry ice
Whole Blood	2 mL Fresh blood in EDTA tube	Blue ice
	4 mL Frozen blood in EDTA tube	Dry ice
	2.5 mL Frozen blood in PAXgene tube	Dry ice
	3 mL Frozen blood in Tempus Blood RNA Tube	Dry ice
Bodily Fluids	500 μL (gDNA)	Blue ice
Bodily Fluids	500-10,000 μL (cell free DNA)	Dry ice

! For research purposes only, not intended for clinical diagnosis, treatment, or individual health assessments.

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