Reduced Representation Bisulfite Sequencing (RRBS)
What Is RRBS
Reduced representative bisulfite sequencing (RRBS-Seq) is a protocol that uses one or more restriction enzymes on genomic DNA to produce sequence-specific fragmentation. The fragmented genomic DNA is then treated with bisulfite and sequenced. This is the method of choice for studying specific regions of interest. It is particularly effective where methylation is high, such as in promoters and repetitive regions. Now, CD Genomics provides you with a variety of WGBS service to meet your research needs.
Specific WGBS Approaches We Offer
| RRBS | Analysis Content | Instructions |
| Bacis Analysis | 1. Quality assessment of sequencing data 2. Comparison with reference genome 3. MSPI digestion and Bisulfite transformation rate quality control 4. Calling of methylation sites 5. Methylation distribution mapping analysis 6. Differential methylation DMR analysis between groups 7. DMG analysis of differentially methylated genes 8. Multiple sample clustering analysis |
Filter out low quality data to ensure data quality Comparative rate and coverage analysis MSPI digestion efficiency >95%; Bisulfite conversion rate >99% Assess cytosine methylation status Methylation in genome, functional elements. Distribution on the genome, functional elements Finding DMR and annotation DMG enrichment analysis of GO, KEGG PCA analysis of methylation change patterns in multiple samples |
| Senior Analysis | 9. Multi-omics integration association analysis 10. Other customized analysis |
For example, association analysis with transcriptome Combined with the subject background highlights mining |
Technology Process
Services Advantages
- Enhanced Double RRBS
Higher CpG coverage than standard RRBS while being more cost-effective than WGBS (ideal for pilot studies).
- High Sequencing Depth
Greater than 10X sequencing depth generating ~ 20 million reads per library.
Our Capabilities
- Cost-effective: lower cost of DNA methylation sequencing compared to WGBS.
- End-to-end package: from sample preparation to data analysis.
- Bioinformatics: Customized bioinformatics analysis, including but not limited to raw data quality control, sequence alignment, methylation calling, differential methylation analysis and clustering of differentially methylated regions (DMRs).
- Fast turnaround time. 4~8 weeks from sample submission.
Sample Requirements
- We currently accept DNA, cell, and tissue samples for our Reduced Representation Bisulfite Sequencing service. Proper sample preparation along with the appropriate quality control methods on your end will allow the greatest chance for assay success.
- DNA: ≥ 3μg for common samples, ≥ 5 μg for FFPE samples
- DNA concentration: ≥ 50 ng/μL
- Purity: OD260/280 = 1.8-2.0 No degradation, no protein or RNA contamination
Service Process
- CD Genomics have professional after-sales service: which has been established to provide customers with efficient, fast, practical and feasible solutions.
- CD Genomics has in-depth cooperation with many biology companies and research institutes, and has extensive experience in providing them with high-quality services.
Our Features
Scientific Program Design
From project proposal, sample processing, library sequencing, to data analysis; each project requires professional and valuable advice; timely and efficient communication to ensure high quality research results.
Deliverables
1. Related experimental results raw data
2. Experimental report
3. Data analysis
4. Image and result analysis
5. Bioinformatics Analysis
Why Choose Us?
At CD Genomics, our goal is to apply innovative technologies to the analysis of genetic variation and function, making possible research that would have been unthinkable even a few years ago. We offer innovative, flexible and scalable solutions that can meet the needs of our customers. We strive to meet this challenge as a global company that places a high priority on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality. If you would like to know more about this service, please feel free to contact us.