Epigenetics, the study of heritable changes in gene expression without alterations to the DNA sequence, plays a crucial role in various biological processes, including development, disease progression, and environmental response. Among the epigenetic modifications, DNA methylation, the addition of a methyl group to cytosine residues, is a key mechanism regulating gene expression. Understanding DNA methylation dynamics and its dysregulation can provide valuable insights into molecular mechanisms underlying biological phenomena.

CD Genomics, a pioneering genomics company offers an advanced and comprehensive DNA methylation microarray service including human methylation microarray and mouse DNA methylation microarray service. Leveraging cutting-edge technology and expertise, this service empowers researchers to unravel the intricate DNA methylation landscape in mouse genomes and elucidate its functional implications.

Our Mouse DNA Methylation Microarray Service

High-Throughput Profiling: Our service enables high-throughput analysis, allowing for the simultaneous assessment of multiple samples. This scalability ensures efficient and cost-effective DNA methylation profiling, particularly when working with a large number of samples.

Sample Preparation and Bisulfite Conversion: We provide guidance and support for sample collection and DNA extraction to ensure high-quality and pure DNA suitable for subsequent analysis. The extracted DNA is then subjected to bisulfite conversion, a process that selectively converts unmethylated cytosine residues to uracils while leaving methylated cytosines unchanged.

Microarray Hybridization and Scanning: The bisulfite-converted DNA is hybridized to the custom-designed microarray, allowing for the detection and quantification of the methylation status of specific CpG sites. The microarray is scanned using state-of-the-art technology to capture the signals generated by the methylated and unmethylated probes.

Bioinformatics Analysis: Our service includes comprehensive bioinformatics analysis to extract meaningful insights from the microarray data. This analysis involves quality control, normalization, statistical analysis, and the identification of differentially methylated regions (DMRs) and CpG sites. Gene ontology and pathway analysis are performed to uncover the biological processes and pathways associated with the identified DNA methylation patterns.

Detailed Reporting and Interpretation: We provide customized reports summarizing the findings of the analysis. These reports include detailed information on differentially methylated CpG sites and regions, as well as gene ontology and pathway analysis results. Our team of experts is available to interpret the results and provide additional insights into the biological implications of the DNA methylation patterns observed.

Mouse DNA Methylation Microarray Advantages

Genome-wide coverage: Provides comprehensive analysis of DNA methylation patterns across the mouse genome.

High-resolution analysis: Allows for precise measurements at single nucleotide resolution, providing detailed information about methylation status.

Cost-effective profiling: Enables large-scale DNA methylation analysis in a cost-efficient manner.

Efficient and time-saving workflow: Streamlines the experimental process, eliminating the need for laborious steps such as bisulfite conversion and targeted sequencing.

Reproducibility and standardization: Offers high reproducibility and standardized conditions, ensuring consistent and reliable results.

Data integration and comparative analysis: Facilitates comparison of multiple samples on the same microarray, enabling the identification of differentially methylated regions and sites.

Mouse DNA Methylation Microarray Service Workflow

CD Genomics' Mouse DNA Methylation Microarray Service entails a streamlined workflow that encompasses various steps, from sample preparation to data analysis:

Data Analysis

The bioinformatics analysis conducted by CD Genomics as part of the Mouse DNA Methylation Microarray Service is pivotal in transforming raw data into valuable biological insights. Leveraging advanced computational tools and robust algorithms, this analysis provides a comprehensive understanding of DNA methylation dynamics and its potential implications.