DNA methylation is an important epigenetic marker information, and obtaining genome-wide methylation level data for all C loci is important for epigenetic spatio-temporal specificity studies. genome-wide DNA methylation sequencing is based on a new generation high-throughput sequencing platform, combined with genome-wide Bisulfite processing and bioinformatics data analysis technology to perform low-cost, high-efficiency and high-accuracy mapping of genome-wide DNA methylation levels.

Sequencing Entire Samples-Without Losing Information

Many methods take advantage of the high quality and sensitivity of NGS for methylation analysis. Most methods rely on heavy sulfite conversion of DNA to detect unmethylated cytosines. During library preparation, the heavy sulfite converts unmethylated cytosine to uracil. The converted bases are identified as thymine in sequencing (after PCR) and the proportion of methylated cytosines is determined by sequence counting. Heavy sulfite conversion sequencing can be accomplished by targeted methods such as amplicon methylation sequencing (Methyl-seq), or whole genome heavy sulfite sequencing (WGBS). In addition, alternative methods such as TAB-Seq and OxBS also utilize NGS to identify hydroxymethylation (5-hmC) and perform methylation (5-mc) analysis.

Specific Genome-wide DNA methylation Approaches We Offer

• Whole Genome Bisulfite Sequencing (WGBS)
• Reduced Representation Bisulfite Sequencing (RRBS)
• MethylRAD-Seq
• MeDIP Sequencing
• DNA 6mA Sequencing
• MRE-Seq Service
• Human DNA Methylation Microarray Service
• Mouse DNA Methylation Microarray Service
• Long Read Sequencing DNA Methylation Service

Genome-wide DNA methylation Sequencing Route

Services Advantages

• Discover methylation patterns in all CpG, CHH and CHG regions across the genome
• Captures full sample diversity with a small amount of DNA
• View the methylation of almost every cytosine in the genome of most species

Sample Requirements

• Sample purity: OD 260/280 values should be between 1.8 and 2.0; RNA should be removed cleanly
• Sample concentration: minimum concentration not less than 50ng/µl.
• Total sample volume: not less than 6µg per sample
• Sample solvent: should be dissolved in H20 or TE (pH 8.0)
• Sample transport: DNA transported at low temperature (-20°C); seal the tube mouth with parafilm during transport to prevent contamination.

Service Process

Our Features

Deliverables

• Base type distribution
• Chromosome level methylation distribution mapping
• DMR-associated gene metabolic pathway analysis
• Annotation of hypermethylated CGI regions
• DMR-related GO gene classification annotation
• 5mC detection

Why Choose Us?

CD Genomics is a company that provides professional and comprehensive genome-wide DNA methylation analysis service. We have years of experience to meet your specific project needs in using epigenomics research to add value to your research projects. CD Genomics can provide you with personalized solutions to help you thrive every step of the way around your interest in your workflow. If you would like to know more about this service, please feel free to contact us.