DNA 6mA Sequencing

DNA methylation is the most basic form of epistatic modification. In eukaryotes, the most common methylation modification is 5-methylcytosine cytosine (5mC). In contrast, in prokaryotes, N6-methyladenine (6mA,or m6A) is the most prevalent DNA modification, where a methyl group is added to the 6th position of the adenine ring. The most studied prokaryotic DNA is 6mA. Now, CD Genomics provides you with a variety of DNA 6mA sequencing service to meet your research needs.  

Specific DNA 6mA Sequencing Approaches We Offer

Methods

6mA DNA immunoprecipitation followed by deep sequencing (6mA DIP-Seq) was developed based on an existing method for detecting adenosine methylation in RNA. In this protocol, genomic DNA is extracted, fragmented, and then the DNA containing 6mA is pulled down with an antibody that recognizes 6mA in the genomic DNA. After subsequent washing, the DNA fragment without 6mA is removed and the fragment containing 6mA is eluted from the antibody for further processing for subsequent analysis.

Scientific program design

Data Analysis

1. Identification of DNA methylation enrichment peaks

2. Annotation of DNA methylation region enrichment peaks

3. Distribution of DNA methylation enrichment peaks in genes

4. Identification of differentially methylated DNA regions (DMRs) and annotation

5. GO (Gene Ontology) and signaling pathway analysis of differentially methylated DNA regions

6. DNA methylation visualization

Services Advantages

• Optimized experimental process

6mA-IP enrichment efficiency is the key to data quality. 6mA-IP experiments use pre-validated commercial antibodies and a carefully optimized experimental process, with high efficiency and specificity

• Strict quality control

Each key step of the experiment is subject to strict quality control to ensure the quality of the experiment throughout the whole process, ensuring that customers get high quality data

Our Capabilities

• Customers only need to provide genomic DNA of relevant samples, CD Gemonics provides a complete service flow from 6mA-IP enrichment, library preparation, up-sequencing to data analysis
• CD Genomics has a strong bioinformatics team that can meet the needs of customers for in-depth data analysis.

Sample Requirements

• Sample type: DNA samples without degradation and without RNA contamination (for all species with reference genome).
• Sample requirement: not less than 10ug
• Sample concentration: not less than 50ng/ul
• Sample transport: samples are placed in 1.5mL Eppendorf tubes, sealed with sealing film and transported on dry ice, DNA can be transported in ice packs.
• Sample preservation: cell samples or fresh tissue blocks cut into pieces and stored at -80℃ after freezing in liquid nitrogen; DNA samples can be stored at -20℃ for a short period of time to avoid repeated freezing and thawing.

Service Process

Deliverables

1. Related experimental results raw data

2. Experimental report

3. Data analysis

4. Image and result analysis

5. Bioinformatics analysis results

6. Details in DNA 6mA sequencing for your writing

Our Features

Why Choose Us?

CD Genomics provides professional gram-negative bacterial outer membrane construction service. We will meet the specific needs of customers on time and on budget. CD Genomics relies on world-class technical expertise, we provide customers with the highest quality one-stop service, including the development of solutions according to different scientific research needs. If you would like to know more about this service, please feel free to contact us.