Understanding DNA methylation is key to decoding how genes are turned on and off—and why things go wrong in disease. This crucial epigenetic mechanism, which involves adding a methyl group to cytosine bases in CpG sites, influences everything from early development to cancer progression. Abnormal methylation patterns have been strongly associated with conditions such as cardiovascular disease, neurodegeneration, and various cancers.
To support cutting-edge research in these areas, CD Genomics offers a high-throughput Human DNA Methylation Microarray Service. This platform delivers single-nucleotide resolution across the methylome, allowing researchers to pinpoint even subtle epigenetic changes.
Available DNA methylation microarray
| Microarray | Species | Coverage | Platform |
|---|---|---|---|
| Infinium Human Methylation 850K |
Human | Over 850,000 methylation sites per sample at single-nucleotide resolution. | Illumina |
| Infinium Human Methylation 935K |
Human | Detects over 935,000 CpG sites, covering CpG islands, promoters, coding regions, and enhancers comprehensively. | Illumina |
| Infinium Methylation Screening Array 270K |
Human | It covers approximately 270,000 methylation sites,with core applications in specific disease cohort research and extensive health screenings. Ensuring high-level precision, this microarray has elevated the single array detection throughput by 48 samples, a six-fold increase from the Infinium MethylationEPIC v2.0, thereby achieving higher throughput and lower costs. | Illumina |
Understanding DNA Methylation
Epigenetics, the study of heritable changes in gene expression that do not involve alterations to the DNA sequence itself, has emerged as a significant field in biological research. One of the crucial mechanisms underlying epigenetic regulation is DNA methylation. DNA methylation plays a pivotal role in gene expression, development, and disease progression.
DNA methylation is a chemical modification that occurs predominantly on cytosine residues within CpG dinucleotides. CpG islands, which are regions of high CpG density, are often found in the promoter regions of genes. Methylation of these CpG islands is associated with gene silencing, thereby influencing gene expression patterns. Aberrant DNA methylation has been implicated in various diseases, including cancer, neurological disorders, and cardiovascular diseases. Therefore, studying DNA methylation patterns is crucial for unraveling the molecular mechanisms underlying disease development and progression.
The Importance of DNA Methylation Microarrays
DNA methylation microarrays are powerful tools that enable high-throughput profiling of DNA methylation patterns across the genome. These microarrays utilize specialized probes that can specifically recognize methylated or unmethylated DNA sequences. By comparing the signal intensities from these probes, researchers can determine the methylation status of CpG sites throughout the genome. This technology allows for the identification of differentially methylated regions associated with specific diseases or biological processes.
CD Genomics Human DNA Methylation Microarray Service
CD genomics provides robust methylation profiling microarray service with extensive coverage of CpG islands, genes, and enhancers to facilitate comprehensive epigenetic studies. This service leverages cutting-edge microarray platforms and advanced bioinformatics analysis to provide researchers with high-quality, reliable data for their studies.
High-throughput Methylation Profiling Service
CD Genomics' Human DNA Methylation Microarray Service offers high-throughput methylation profiling, allowing researchers to analyze thousands of CpG sites simultaneously. By utilizing microarray platforms with high-density probe coverage, this service enables comprehensive genome-wide methylation profiling, providing researchers with a detailed understanding of DNA methylation patterns.
Customized Experimental Designs
To meet the diverse needs of researchers, CD Genomics offers customized experimental designs for the Human DNA Methylation Microarray Service. Researchers can choose from a wide range of options, including sample types, array formats, and analysis strategies, tailored to their specific research objectives. This flexibility ensures that researchers can obtain the most relevant and informative data for their studies.
Advanced Bioinformatics Analysis
CD Genomics' Human DNA Methylation Microarray Service includes advanced bioinformatics analysis to maximize the value of the generated data. The service employs sophisticated algorithms and analytical pipelines to identify differentially methylated regions, perform pathway enrichment analyses, and generate comprehensive reports. These analyses provide valuable insights into the functional implications of DNA methylation changes and aid in the interpretation of the results.
Applications of Human DNA Methylation Microarray Service
The versatility of CD Genomics' Human DNA Methylation Microarray Service makes it applicable to various research areas. Here are some notable applications:
Cancer Research
Cancer is characterized by widespread alterations in DNA methylation patterns. CD Genomics' Human DNA Methylation Microarray Service allows researchers to investigate aberrant DNA methylation events associated with tumorigenesis. By comparing cancer samples with normal controls, researchers can identify cancer-specific DNA methylation changes that may serve as diagnostic or prognostic markers. Furthermore, these studies can shed light on the underlying molecular mechanisms driving cancer development and aid in the development of targeted therapies.
Developmental Biology
During development, DNA methylation plays a critical role in regulating gene expression patterns and cellular differentiation. CD Genomics' Human DNA Methylation Microarray Service enables researchers to study dynamic changes in DNA methylation during embryonic development, tissue differentiation, and organogenesis. By profiling DNA methylation patterns at different developmental stages, researchers can uncover key regulatory elements and elucidate the epigenetic mechanisms governing normal development.
Environmental Epigenomics
Environmental factors can influence DNA methylation patterns, leading to long-lasting effects on health and disease susceptibility. CD Genomics' Human DNA Methylation Microarray Service facilitates the study of environmental epigenomics by enabling the identification of DNA methylation changes associated with specific environmental exposures. These studies provide insights into the molecular mechanisms underlying environmental effects and contribute to the development of personalized medicine strategies.
Workflow
Service Advantages
Simple Workflow: The service eliminates the need for cumbersome immunoprecipitation steps.
Direct Detection of Methylation Sites: It accurately detects specific methylated sites rather than methylated regions.
Customizable Chip Design: The service allows for flexible customization of personalized chips to meet specific research needs.
High Throughput: It has the capacity to simultaneously analyze 12 or more samples in a single run, enabling efficient large-scale studies.
