acRIP-seq

Chemical modifications of RNA have emerged as a new mechanism for the posttranscriptional regulation of gene expression. While the majority of RNA modifications are methylation events, only a single acetylated ribonucleoside has been described in eukaryotes, occurring at the N4-position of cytidine (N4acetylcytidine, ac4C). In all cases, ac4C production has been catalyzed by the N-acetyltransferase 10 (NAT10) enzyme or its homologs. Studies have indicated that ac4C is associated with RNA stability and translation efficiency. CD Genomics uses acRIP-Seq technology to comprehensively analyze ac4C in the transcriptome.

Figure 1. NAT10 catalyzes cytidine acetylation (Arango,2019)

Specific acRIP-seq Approaches We Offer

Methods

• PolyA captures mRNA or rRNA depletion
• RNA fragmentation (about 100nt)
• RNA immunoprecipitation with anti-ac4C antibodies (or Isotypic IgG control)
• Recovery and purification of acetylated RNA
• PCR amplification and construction of library

Our O8G-Seq are available for a wide range of sequencing applications:

• Complete transcriptome sequencing of ac4C
• Sequencing of ac4C cyclic RNA
• ac4C LnRNA sequencing
• ac4C mRNA sequencing

Data Analysis

Service Advantages

• Specific enrichment and detection of RNA fragments acetylation by ac4C
• RNA ac4C acetylation detection in the whole transcriptome
• Targeted detection of ac4C acetylation of different types of RNA molecules

Our Capabilities

• Wide detection range: Whole-transcriptome wide RNA ac4C acetylation profiling.
• Flexible sample requirements: Cells, tissues or RNA are acceptable
• Specialized bioinformatics analysis: Strong bioinformatic team, professional ac4C acetylation data analysis.

Sample Requirements

• Cell samples

The number of cells shall not be less than 2×10⁷.

• Fresh tissue samples

The quantity of the tissue samples should be between 500 mg - 1g.

• RNA samples

The quantity of RNA sample should be no less than 30 μg, RNA purity: OD260/280 = 1.8~2.2; OD260/230 ≥ 1.5; RNA quality: 28S:18S ≥ 1.5, RIN ≥ 7.

Service Process

Deliverables

• Related experimental results raw data
• Experimental report
• Data analysis
• Image and result analysis
• Bioinformatics analysis results
• Details in acRIP-seq for your writing

Our Features

Why Choose Us?

CD Genomics is a company that provides professional and comprehensive acRIP-seq services. We have years of experience to meet your specific project needs in using epigenomics research to add value to your research projects. CD Genomics can provide you with personalized solutions to help you thrive every step of the way around your interest in your workflow. If you would like to know more about this service, please feel free to contact us.

Reference

1. Arango, Daniel, et al. Immunoprecipitation and sequencing of acetylated RNA. Bio-protocol 9.12 (2019): e3278-e3278.