acRIP-seq
Chemical modifications of RNA have emerged as a new mechanism for the posttranscriptional regulation of gene expression. While the majority of RNA modifications are methylation events, only a single acetylated ribonucleoside has been described in eukaryotes, occurring at the N4-position of cytidine (N4acetylcytidine, ac4C). In all cases, ac4C production has been catalyzed by the N-acetyltransferase 10 (NAT10) enzyme or its homologs. Studies have indicated that ac4C is associated with RNA stability and translation efficiency. CD Genomics uses acRIP-Seq technology to comprehensively analyze ac4C in the transcriptome.
Figure 1. NAT10 catalyzes cytidine acetylation (Arango,2019)
Specific acRIP-seq Approaches We Offer
Methods
- PolyA captures mRNA or rRNA depletion
- RNA fragmentation (about 100nt)
- RNA immunoprecipitation with anti-ac4C antibodies (or Isotypic IgG control)
- Recovery and purification of acetylated RNA
- PCR amplification and construction of library
Our O8G-Seq are available for a wide range of sequencing applications:
- Complete transcriptome sequencing of ac4C
- Sequencing of ac4C cyclic RNA
- ac4C LnRNA sequencing
- ac4C mRNA sequencing
Data Analysis
| Basic analysis | Peak Calling analysis Visualization of Peak Statistical analysis of Peak Feature of ac4C Differential Peak analysis Length distribution of acetylation sites and motif analysis GO and Pathway analysis results of differential peak related genes |
| Advanced Analytics | Association between RNAseq expression difference and ac4C modification difference Association Analysis of mRNA Variable Splicing and ac4C modification Difference |
Service Advantages
- Specific enrichment and detection of RNA fragments acetylation by ac4C
- RNA ac4C acetylation detection in the whole transcriptome
- Targeted detection of ac4C acetylation of different types of RNA molecules
Our Capabilities
- Wide detection range: Whole-transcriptome wide RNA ac4C acetylation profiling.
- Flexible sample requirements: Cells, tissues or RNA are acceptable
- Specialized bioinformatics analysis: Strong bioinformatic team, professional ac4C acetylation data analysis.
Sample Requirements
- Cell samples
The number of cells shall not be less than 2×107.
- Fresh tissue samples
The quantity of the tissue samples should be between 500 mg - 1g.
- RNA samples
The quantity of RNA sample should be no less than 30 μg, RNA purity: OD260/280 = 1.8~2.2; OD260/230 ≥ 1.5; RNA quality: 28S:18S ≥ 1.5, RIN ≥ 7.
Service Process
Deliverables
- Related experimental results raw data
- Experimental report
- Data analysis
- Image and result analysis
- Bioinformatics analysis results
- Details in acRIP-seq for your writing
Our Features
- CD Genomics will complete your project on time and efficiently. We have professional after-sales service.
- CD Genomics works with scientists from many biotechnology companies. We have extensive knowledge and experience to provide quality assurance services.
Why Choose Us?
CD Genomics is a company that provides professional and comprehensive acRIP-seq services. We have years of experience to meet your specific project needs in using epigenomics research to add value to your research projects. CD Genomics can provide you with personalized solutions to help you thrive every step of the way around your interest in your workflow. If you would like to know more about this service, please feel free to contact us.
Reference
- Arango, Daniel, et al. Immunoprecipitation and sequencing of acetylated RNA. Bio-protocol 9.12 (2019): e3278-e3278.