DNA Hydroxymethylation Analysis Service
Hydroxymethylation 5hmC is the sixth base on the mammalian genome and plays an important role in development, aging, neurodegenerative diseases, complex diseases and tumorigenesis. DNA hydroxymethylation is a new DNA modification that has been discovered in recent years and is rapidly becoming a research hotspot. As research progressed, it was found that heavy sulfite sequencing, previously considered the standard for detecting DNA methylation, could not distinguish between DNA methylation (5mC) and DNA hydroxymethylation (5hmC).
DNA Hydroxymethylation Analysis Service Approaches We Offer
- hMeDIP-seq
- hmC Seal-seq
- oxBS-seq
- Targeted DNA hydroxymethylation analysis (oxTBS)
Solutions
CD Genomics offers DNA hydroxymethylation analysis service, our technology can not only accurately detects DNA methylation and excludes the effects of DNA hydroxymethylation, but also allows the combination of dual libraries with simultaneous single-base resolution to accurately detect DNA hydroxymethylation.
| Sequencing data quality assessment | Overview of whole gene hydroxymethylation levels | Identification and statistics of differential hydroxymethylation sites (DhMC) |
|---|---|---|
| 1. Oxidation efficiency and conversion efficiency evaluation 2. Original downstream data quality control 3. Comparison quality assessment 4. Sequencing depth and coverage assessment |
1. Display of genome-wide hydroxymethylation profiles for each sample 2. Average hydroxymethylation level statistics 3. distribution of whole gene hydroxymethylation levels 4. Comparison of the distribution of hydroxymethylation levels by samples 5. Characteristics of hydroxymethylation level of each gene element 6. Hierarchical clustering based on genome-wide hydroxymethylation profiles 7. Principal component analysis based on genome-wide hydroxymethylation profiles |
1. Identification of differential hydroxymethylation sites (DhMC) 2. annotation of DhMC 3. Statistics of DhMC modified genes 4. Distribution of DhMC on chromosomes 5. Distribution of DhMC on gene elements 6. Functional enrichment analysis of DhMC-modified genes |
Services Advantages
- Single-base detection of DNA hydroxymethylation modifications.
- Multiplex quality control standards to detect oxidation efficiency and Bisulfite conversion rate.
- Low experimental preference and high reproducibility (R2 > 0.98).
- Multiple sequencing applications can be met with.
Sample Requirements
- Total amount:DNA>2ug,tissue sample>100mg,sample concentration>50ng/ul,whole blood sample>2ml,cell sample;
- Sample status: whole blood samples should be used in normal EDTA anticoagulation tubes, avoid using heparin anticoagulation tubes; cell samples should be live cell frozen samples; purity: OD260/280=1.8-2.0;
- Sample integrity: no significant degradation of DNA and protein contamination, as judged by gel electrophoresis; recommended data volume: 10g raw data.
Service Process
Our Features
- CD Genomics has a group of technical experts specializing in DNA hydroxymethylation analysis service. Our extensive research and technical experience can help our customers solve their biofilm technical problems.
- CD Genomics has a proven, advanced DNA hydroxymethylation technology platform that is constantly being improved to ensure that we can provide accurate, sensitive, and rapid analytical results for our customers.
Deliverables
1. Related experimental results raw data
2. Experimental report
3. Data analysis
4. Image and result analysis
5. Bioinformatics analysis results
6. Details in DNA hydroxymethylation analysis service for your writing
Why Choose Us?
CD Genomics is a company that provides professional and comprehensive DNA hydroxymethylation analysis service. We have years of experience to meet your specific project needs in using epigenomics research to add value to your research projects. CD Genomics can provide you with personalized solutions to help you thrive every step of the way around your interest in your workflow. If you would like to know more about this service, please feel free to contact us.