Chromatin Analysis

Chromatin is composed of DNA, histones and non-histones. Understanding the role of histones and transcription factors is critical to understanding the regulation of gene expression. Using ChIP-seq analysis, histone modifications and transcription factor binding can be analyzed on a genome-wide scale to elucidate the control of gene expression in disease or in response to drug therapy. CD Genomics' chromatin analysis offers a variety of chromatin analysis options through ChIP-seq and ATAC-seq.

Methods

• ChIP-chip: ChIP-chip combines chromatin IP with microarray analysis, allowing genome-wide analysis of proteins or modification of the distribution of interest.
• ChIP-Seq: ChIP-seq uses the same chromatin IP program as ChIP-chip; however, it combines it with quantitative next-generation sequencing technology to detect enrichment peaks
• ChIP-exo: A special version of ChIP for very specific mapping of protein of interest (POI) binding sites in the genome by adding a DNA digestion step to ChIP-seq.
• ChIA-PET (Chromatin Interaction Analysis by Double-Ended Tag Sequencing): combines ChIP with Chromatin Conformation Capture (3C) technology to detect when distant DNA regions interact through proteins of interest.

Specific Chromatin Analysis Approaches We Offer

• ChIP-seq
• ATAC-Seq  
• Single-cell ATAC-Seq (scATAC-Seq)
• DAP-Seq Service
• Hi-C Service (Chromosome Conformation Capture, 3C)
• SDNase-Seq
• MNase-seq 
• NOMe-Seq 

Solutions

• ATAC-seq: open chromatin regions

Study genome-wide chromatin accessibility. Identifies open chromatin loci and active regulatory elements such as promoters, enhancers and insulators.

• ChIP-seq/ChIP-qPCR Histone modifications

Post-translational modifications of histones are associated with the regulation of gene expression, making it necessary to study regulatory elements and their interacting proteins, such as active promoters and enhancers, for analysis. Analysis of genome-wide histone modifications by ChIP-seq analysis to understand transcriptional regulation
Active promoter analysis: H3K4me3 enrichment
Inactive promoter analysis: H3K27me3 enrichment
Enhancer analysis: H3K4me1 and H3K27ac enrichment in regulatory regions
Active gene body: H3K36me3

• ChIP-seq/ChIP-qPCR transcription factors

The impact of transcription factor binding was explored by ChIP-seq analysis of a large number of TFs, including
CTCF: transcriptional repressor and insulator activity
p300/CBP: histone acetyltransferase
Pol II, p53, etc.
Epigenetic authors, readers, erasers
Nuclear receptors
Oncogenes

• Custom NGS Services

Low input library from Pico
Specific analysis
Complete custom projects

Services Advantages

• Highly efficient enrichment of target DNA, with positive vs. negative controls at >500.
• High sensitivity and flexibility allow for the preparation of non-barcoded (single duplex) and barcoded (multiplex) DNA libraries.

Sample Requirements

• Cells required for ChIP-seq reaction should be greater than 10million/tube.
• Select fresh tissues with mass greater than 100mg/serving, snap frozen in liquid nitrogen and stored at -80℃. It is recommended that samples be prepared in 2~3 portions.

Service Process

Our Features

Deliverables

• Related experimental results raw data
• Experimental report
• Data analysis
• Image and result analysis
• Bioinformatics analysis results

Why Choose Us?

At CD Genomics, our goal is to apply innovative technologies to genetic and genetic analysis.We offer innovative, flexible and scalable solutions that can meet the needs of our customers. We strive to meet this challenge as a global company that places a high priority on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality. If you would like to know more about this service, please feel free to contact us.