Oxford Nanopore Sequencing Technology

As a leading genomics company, CD Genomics provides next-generation sequencing and bioinformatics services to pharmaceutical and biotech companies, as well as academic and government agencies around the world, relying on advanced sequencing instruments and rich project experience. The next era of DNA sequencing technology, also known as termed long-read sequencing or third-generation sequencing (TGS), plays an increasingly important role in biology. We are offering long-read sequencing services from PacBio and Oxford Nanopore, giving researchers a wide range of cutting-edge sequencing services to suit particular needs at every stage of any projects. With years of experience in long-read sequencing technology, we are able to generate and analyze high quality data using the latest workflows.

Oxford Nanopore Sequencing Technology (ONT)

-Technical Principle of ONT

Nanopore sequencing with Oxford Nanopore Technologies (ONT) systems is able to perform high-throughput long-read sequencing of both DNA and RNA samples, involving genomic DNA, amplified DNA, cDNA, and RNA. Briefly, the nanopore sequencer is based on a novel 'nanopore technology that uses nanopores embedded in an electro-resistant membrane. A voltage is set on the membrane to form an ionic current across the pores. When a DNA molecule tagged with tether proteinis guided and passes through a nanopore, the current variation, producing a characteristic signal, which is mainly influenced by four DNA bases. Then a base-calling algorithm converts the current variation back into the original DNA sequence in real-time.

Fig.1 Schematic representation of a DNA molecule translocating a protein nanopore.

-Outstanding Advantages of ONT

• Long reads with no amplification
  The long read length of over 150 Kbp provides a more comprehensive display of the sequence information of the target gene and facilitates the identification of duplicated gene fragments and structural variants.
• Direct detection of target nucleic acids without PCR amplification
  Enable direct sequencing of epigenetic modifications and RNA.
• Real-time access to sequence information
  Direct detection of the native strand of interest saves a lot of time. Sequencing signals are collected directly when the target nucleic acid passes through the nanopore, and sequencing results can be output in real time.
• Simple library preparation
  No PCR amplification, no fluorescent molecular markers, no optical imaging, extremely fast library preparation process.

-Applications of ONT

ONT directly sequences the native strand of interest, without optics or amplification and its data has been proven improve de novo genome assemblies, structural genomic variant, and so on. ONT has been applied in genomic analysis, identification of pathogens, metagenomic environmental monitoring, food safety monitoring and other aspects of life sciences.

Major ONT Systems are Available at CD Genomics

Based on Oxford Nanopore Sequencing technology, CD Genomics can help researchers and professionals with a wide range of cutting-edge sequencing services at competitive prices. Our clients have direct access to our staff and prompt feedback to their inquiries. If you are interested in our services, please don't hesitate to contact us for more details.

Related Services

• Human Genomics with Long-Read Sequencing
• Animal and Plant Genomics with Long-Read Sequencing
• Microbial Genomics with Long-Read Sequencing
• Transcriptomics with Long-Read Sequencing
• Epigenetics and Methylation Analysis Using Long-Read Sequencing
• Whole-Genome Resequencing with Long-Read Sequencing
• Pre-Made Library Long-Read Sequencing Services
• Long-Read Sequencing Data Analysis Services