Animal/Plant Long Amplicon Sequencing

Long-read amplicon sequencing shows promise as a powerful tool for studying complete genes or genomes of complex and diverse populations. Based on Single Molecule, Real-Time (SMRT) sequencing and its advanced Sequel sequencing system, CD Genomics is proud to offer professional, reliable, and cost-effective animal/plant long amplicon sequencing services to meet the project needs of our clients worldwide. Although the genomes of animals and plants are more complex than those of humans. Our services still allow precise detection and discovery of almost all types of variation in plant and animal genomes, facilitating the identification and functional studies of disease resistance genes associated with disease susceptibility, genotyping studies and breeding decisions, and so on.

Advantages of Long-Amplicon Sequencing using PacBio SMRT Sequencing

• Capable of processing and generating contiguous sequence reads for multiple genes without the need for a DNA fragmentation step.
• Accurate identification and exclusion of off-target signals from homologous sequences in the genome of interest, such as pseudogenes.
• The SMRT sequencing features a context-independent error profile that allows for high-quality consensus reads of the >QV50.
• Cost-efficient and allows for phasing of multiple heterozygous variants and accurate variant calling.

Our Service Workflow

Sample Requirements

1. High-quality nucleic acids: 200ng or more.
2. Since we offer amplicon preparation services, we also accept other samples, such as cell pellets, and other tissue samples.

Data Analysis Contents

-Circular consensus sequence (CCS) analysis.

-Long amplicon analysis (LAA).

-Call variants at every scale, including structural variation (SV), rare single nucleotide polymorphisms (SNPs), insertion-deletion mutations (InDel), copy number variation (CNV), and homopolymer repeats.

-Minor variant analysis.

We Can Help Customers Achieve

• Help identify rare somatic mutations in complex samples.
• Detection and characterization of all types of variants.
• Identification of haplotype-specific markers.
• Analysis of hard-to-sequence regions without regard to size.
• Confirmation of transgene insertion location and verification of gene editing events.

Based on the PacBio Sequel II sequencing platform, the single-molecule real-time sequencing approach allows researchers to analyze genetic variation in specific genomic regions of plants and animals. Long-read sequencing of PCR products (amplicons) allows for accurate identification and efficient characterization of genetic variation. CD Genomics is dedicated to providing fast and reliable long amplicon sequencing and analysis services for humans, animals, plants, and microorganisms. If you are interested in our services, please don’t hesitate to contact us. Discuss your research with one of our expert scientists. We look forward to working with you on your next project.