MethylRAD-Seq
DNA methylation is a common mechanism of epigenetic regulation in eukaryotes. MethylRAD uses one of the Mrr-like enzymes (e.g. FspEI, MspJI, LpnPI, AspBHI, etc.) to perform reduced methylome sequencing for cost-efficient DNA methylation profiling. Similar to affinity-based methods, these enzymes can directly assess the DNA methylation status without the aid of chemical conversion, but with much higher specificity, sensitivity and reproducibility. MethylRAD allows for de novo (reference-free) methylation analysis, extremely low DNA input and adjustment of tag density. MethylRAD ideally suited for large-scale methylation profiling projects.
Figure 1. Schematic overview of the procedure for MethylRAD library preparation (Wang, 2015)
Specific MethylRAD-SeqApproaches We Offer
Methods
- Genomic DNA is digested with the restriction enzyme FspEI, producing 32-bp fragments
- Adaptors with compatible overhangs (selective) are ligated to each end of these fragments
- The constructs are amplified and purified
- Sample-specific barcodes are incorporated in each construct by PCR and further sequencing
Our MethylRAD-Seq are available for a wide range of sequencing applications:
- Reference-based analysis
- De novo methylation analysis
Our bioinformatics analysis services include, but are not limited to:
- Sequencing data quality control
- Comparison with reference genome
- Identified methylated sites
- Length distribution of methylated sites and motif analysis results
- Genome-wide distribution of differentially methylated sites
Service Advantages
- MethylRAD protocol is substantially simpler than previous protocols
- MethylRAD library preparation can use extremely low amounts of input DNA
- MethylRAD allows researchers to adjust the tag density using selective adaptors to maximize sample throughput while minimizing costs
- MethylRAD represents an advisable option for large-scale methylation profiling studies dealing with species with large genomes
Our Capabilities
- Wide detection range: Whole- Genome wide methylated detection.
- High sensitivity: The performance for detecting lowly methylated sites is very encouraging
- de novo methylation analysis: Developed a procedure for de novo MethylRAD analysis
- Specialized bioinformatics analysis: Strong bioinformatic team, professional Nm data analysis.
Sample Requirements
DNA samples
The quantity of DNA sample should be 1 – 200 ng, DNA purity: OD260/280: 1.8~2.0.
Service Process
Deliverables
- Related experimental results raw data
- Experimental report
- Data analysis
- Image and result analysis
- Bioinformatics analysis results
- Details in MethylRAD sequencing for your writing
Our Features
- CD Genomics will complete your project on time and efficiently. We have professional after-sales service.
- CD Genomics works with scientists from many biotechnology companies. We have extensive knowledge and experience to provide quality assurance services.
Why Choose Us?
CD Genomics is a company that provides professional and comprehensive MethylRAD -seq services. We can conduct directly assess the Whole Genome wide DNA methylation status with low input and provide you satisfactory specificity, sensitivity and reproducibility. If you would like to know more about this service, please feel free to contact us.
Reference
- Wang, Shi, et al. MethylRAD: a simple and scalable method for genome-wide DNA methylation profiling using methylation-dependent restriction enzymes. Open biology 5.11 (2015): 150130.