EpiTYPER DNA Methylation Analysis
DNA methylation plays a profound role in development and health as well as development and progression of disease. The EpiTYPER assay is a region-specific method for the detection and quantitative analysis of DNA methylation that allows performing a high-resolution scan of selected regions. It thus enables a more detailed analysis of single CpGs and the surrounding area and can, in addition to candidate gene methylation analysis, be used to validate CpGs detected by genome wide techniques. EpiTYPER assay detects and quantitatively analyzes DNA methylation using base-specific cleavage of bisulfite-converted genomic DNA and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), which is an accurate, reproducible, and quantitative tool to analyze genomic DNA methylation.
Figure 1. Scheme of the EpiTYPER technique (Kunze, 2018)
Specific EpiTYPER DNA Methylation Analysis We Offer
Methods
- The primers were designed using the EpiTyper assay designer
- Genomic DNA was bisulfite converted using the DNA methylation kit
- PCR amplification using primers located around the CpG site of interest
- The cleavage products are then analyzed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS)
Our EpiTYPER DNA Methylation Analysis are available for a wide range of sequencing applications:
- Targeted DNA methylation
- Genotyping and mutation detection
Our bioinformatics analysis services include, but are not limited to:
- Sequencing data quality control
- Comparison with reference genome
- Identified methylated sites
- Length distribution of methylated sites and motif analysis results
- Genome-wide distribution of differentially methylated sites
Service Advantages
- EpiTyper DNA methylation analysis is a highly quantitative assay
- EpiTyper DNA methylation analysis is a region-specific method
- EpiTyper DNA methylation analysis allows a fast and reproducible targeted quantification of individual CpGs
- EpiTyper DNA methylation analysis allows the simultaneous analysis of multiple CpGs within a specific region
Our Capabilities
- Wide detection range: Whole- Genome wide methylated detection.
- High accuracy: The detection precision allows low quantifying methylation
- Specialized bioinformatics analysis: Strong bioinformatic team, professional methylated data analysis.
Sample Requirements
DNA samples
- The amount of DNA sample should not less than 1 μg, DNA purity: OD260/280: 1.8~2.0.
Service Process
Deliverables
- Related experimental results raw data
- Experimental report
- Data analysis
- Image and result analysis
- Bioinformatics analysis results
- Details in EpiTYPER DNA methylation analysis for your writing
Our Features
- CD Genomics will complete your project on time and efficiently. We have professional after-sales service.
- CD Genomics works with scientists from many biotechnology companies. We have extensive knowledge and experience to provide quality assurance services.
Why Choose Us?
CD Genomics is a company that provides professional and comprehensive EpiTYPER DNA methylation analysis services. We can offer an accurate, reproducible, and quantitative service to analyze genomic DNA methylation. The service allows the analysis of selected CpGs with proven functional relevance of different regions. If you would like to know more about this service, please feel free to contact us.
Reference
- Kunze, Sonja, et al. Quantitative region-specific DNA methylation analysis by the EpiTYPER™ technology. DNA Methylation Protocols. Humana Press, New York, NY, 2018. 515-535.