Targeted Metabolomics Analysis

Metabolome can be described as the complete set of metabolites (molecules with low molecular weight) present within an organism, cell, or tissue. The analysis of gut microbial metabolites will facilitate the understanding of gut physiology and the role of gut microbiota in the gastrointestinal tract. Metabolomics is a promising field for investigating the molecular mechanism of gut microbiota, the general approaches of measuring these molecules are divided into two groups: targeted metabolomics and untargeted metabolomics. Targeted metabolome can determine the content of metabolites in the sample.

Host and intestinal bacteria produce a large number of small molecular metabolites when they participate in the co-metabolism of food or exogenous substances. Among them, there are some molecules that play a key role in the transmission of host cells and intestinal bacteria. Different segments of the intestine have different dominant flora. Each part of the intestine has specific chemical signals for communication between bacterial groups (including food chain, information transmission, gas transmitters, etc.) and host-bacterial communication. These substances include low molecular weight metabolites, peptides, proteins, etc., which play an important role in the host.

CD Genomics, as a preeminent corporation that specializes in multi-omics and equipped with state-of-the-art techniques as well as well-experienced scientists, provides one-stop targeted metabolomics services for your gut microbiota research.

What We Can Provide

Absolute quantitative detection of microbiota-host metabolites based on gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography-mass spectrometry (HPLC-MS) technology platforms. The types of substances we can detect include but are not limited to the following table. If you want to detect other metabolites that are not in the table, you can contact us.

Advantages of Targeted Metabolomics Service

• Advanced platform: Ultra-high resolution, ultra-high sensitivity and fast scanning speed QE HF mass spectrometry platform is used for detection, with large throughput and more compound information can be identified.
• High-quality database: The primary and secondary mass spectra in the database are all real spectra detected by the high-quality, high-resolution, high-resolution Orbitrap mass spectrometer using standard products. The compound identification accuracy is high.
• Personalized service: Have a professional information analysis team to analyze the data results. Personalized information analysis can also be carried out according to customer needs.

Sample Requirements

• Blood ≥ 100 µL
• Urine ≥ 100 μL
• Tissue ≥ 50 mg
• Fresh stool ≥ 100 mg
• Freeze-dried stool ≥ 10 mg
• Cell ≥1*10⁷

Duplicate samples:

• Animal samples (various tissues, plasma) ≥6 replicates per group
• Microbial samples ≥ 3 replicates per group
• Cell sample ≥ 3 replicates per group
• Clinical samples (serum, urine, various tissues, etc.) ≥10 cases in each group

Data Analysis

• Raw data
• Data preprocessing
• Data quality control
• Metabolite annotation

*For Research Use Only. Not for use in diagnostic procedures.