The Magnetic Viral DNA&RNA Kit is specifically designed for the rapid and high-throughput purification of viral DNA/RNA from serum, plasma, and other sources. Nucleic acids (DNA / RNA) attach to the silica surface of the magnetic particles, free of proteins, nucleases, and other impurities, using a special magnetic beads technology and a particular buffer solution. It has several advantages, including the ability to purify high-quality, ready-to-use viral DNA and RNA in 45 minutes and the absence of phenol or chloroform extraction. This scheme can easily be modified to work with automated high-throughput nucleic acid purification systems from Eppendorf, Thermo, and Beckman, among others. Purified DNA/RNA can be used in downstream applications such as PCR, RT-PCR, real-time PCR, NGS, and so on. Dry storage at room temperature (15-25°C) is recommended for this kit.
Storage:
This Kit can be stored dry at room temperature(15-25°C).
Components:
Specifications:
| Features | Rapid purification of high quality, ready-to-use viral DNA and RNA within 45 minutes. No phenol / chloroform extraction. This system can be easily adapted with automated high-throughput nucleic acid purification systems such as Eppendorf, Thermo, and Beckman, etc. |
| Application | The purified DNA/RNA is ready for use in downstream applications such as PCR, RT-PCR, realtime PCR, NGS, etc. |
| Species Category | Can be used to isolate nucleic acids from human and animal viruses. |
| Sample type | 200 μl plasma, serum, other cell-free materials; 140 μl Swab preservation solution, VTM |
Virus Extraction Kits
| Cat. No. | Product Name | Specs | |
|---|---|---|---|
| DCE008 | CD Viral DNA&RNA Kit | 50 | View product page |
Positive Control
| Cat. No. | Product Name | Specs | |
|---|---|---|---|
| MD002 | CD-2019-nCoV-abn | 1ml / 5ml / 10ml | View product page |
| MD003 | CD-2019-nCoV-abEN | 1ml / 5ml / 10ml | View product page |
One Step qRT-PCR Kits
| Cat. No. | Product Name | Specs | |
|---|---|---|---|
| QRT001 | CD One Step qRT-PCR SYBR Green Kit | 250 / 100 | View product page |
| QRT002 | CD One Step qRT-PCR Probe Kit | 250 / 100 | View product page |
NGS Metagenome Preparation Kits
| Cat. No. | Product Name | Specs | |
|---|---|---|---|
| DP001 | CD Universal DNA Library Prep Kit for Illumina | 24 / 96 / 24(PCR-free) / 96(PCR-free) | View product page |
| DP002 | CD NEXT DNA Library Prep Kit for Illumina | 24(50ng) / 96(50ng) / 24(5ng) / 96(5ng) / 24(1ng) / 96(1ng) | View product page |
| CB001 | CD DNA Clean Beads | 5ml / 60ml / 450ml | View product page |
| DSI001 | CD DNA Adapters S1-S2 for Illumina | 48 / 192 / 48 / 192 | View product page |
| DSI002 | CD DNA Adapters S3-S6 for Illumina | 192 / 192 / 192 / 192 | View product page |
| DDI001 | CD Multiplex Oligos S1 for Illumina | 192 | View product page |
| DDI002 | CD Multiplex Oligos S2 for Illumina | 192 | View product page |
| DDI003 | CD NEXT Index Kit S1 for Illumina | 192 | View product page |
| DDI004 | CD NEXT Index Kit S2 for Illumina | 768 | View product page |
| DDI005 | CD NEXT Index Kit S3 for Illumina | 192 / 192 / 192 / 192 | View product page |
Please add isopropanol to Buffer RLCK before use, the volume as described on the bottle. Add ethanol (96-100%) to Buffer PWC and PWE before use, the volume as described on the bottle.
- Add 200 μl of plasma/serum/ lymph into a centrifuge tube (not provided) (Equilibrate the samples to room temperature.); or take 140 μl Swab preservation solution or VTM add 0.9% sodium chloride solution to 200μl.
- Add 15μl MagAttract Suspension G into the centrifuge tube.
- Pipet 20 μl Proteinase K into the centrifuge tube.
- Add 300 μl Carrier RNA working solution (mixture of Buffer RLCK and Carrier RNA solution, please refer to table 1). Close the cap and mix by pulse-vortex for 10 s.
- Incubate at room temperature for 10min, mix the mixture upside down for 10s per 3min, ensure nucleic acids (DNA / RNA ) binds to the silica surface of the magnetic particles sufficiently. Briefly centrifuge the 1.5 ml tube to collect drops from the inside of the lid.
- Place the centrifuge tube on a magnetic separation device for 1 min. Allow the magnetic particles to completely clear from the solution and then discard the supernatant carefully.
- Remove the centrifuge tube from the magnetic separation device. Add 500 μl Buffer PWC (ensure that ethanol (96-100%) has been added into Buffer PWC before use), and mix by pipetting or vortex.
- Place the centrifuge tube on a magnetic separation device for 1 min. Allow the magnetic particles to completely clear from the solution and then discard the supernatant carefully.
- Remove the centrifuge tube from the magnetic separation device. Add 500 μl Buffer PWE (ensure that ethanol (96-100%) has been added into Buffer PWE before use), and mix by pipetting or vortex.
- Place the centrifuge tube on a magnetic separation device for 1 min. Allow the magnetic particles to completely clear from the solution and then discard the supernatant carefully.
- Repeat step 9 and 10, clear the solution as possible.
- Place the centrifuge tube on the magnetic separation device for 5-10 min at 56°C.
- Remove the centrifuge tube from magnetic separation device. Add 100 μl RNase-Free ddH2O to elute nucleic acids. Mix by pipetting or vortex and incubate at 56°C for 5 min.
- Place the centrifuge tube on the magnetic separation device for 2 min until all the magnetic particles are cleared from the solution. Transfer the supernatant containing purified nucleic acids to a new centrifuge tube and stores it.
