The Viral DNA&RNA Kit is intended to isolate viral DNA/RNA from plasma, serum, and other cell-free materials in a fast, simple, and cost-effective miniprep technique. The Kit is ready to use without phenol/chloroform and is based on silica-membrane technology and a unique buffer system. The Kit's carrier RNA enhances viral RNA binding to the membrane, especially in low-titer specimens. A small volume of RNase-free ddH2O elutes high-quality viral DNA/RNA. Purified DNA/RNA can be used in PCR, RT-PCR, real-time PCR, nest PCR, and other downstream applications. The following are examples of acceptable sample types: 140 l Swab preservation solution, VTM; 200 l plasma, serum, and other cell-free materials.
Storage:
This Kit can be stored dry at room temperature(15-25°C).
Components:
Specifications:
| Features | High quality viral DNA/RNA can be purified from samples of various virus including HBV, HPV, HCV and enteroviruses within one hour. No phenol/chloroform extraction. |
| Application | Virus genotyping research Virus epidemiologic study Virus infectious diseases analysis and drug resistance analysis |
| Species Category | Can be used to isolate nucleic acids from human and animal viruses. |
| Sample type | 200 μl plasma, serum, other cell-free materials; 140 μl Swab preservation solution, VTM |
Related Products
Virus Extraction Kits
| Cat. No. | Product Name | Specs | |
|---|---|---|---|
| DCE102 | CD Magnetic Viral DNA&RNA Kit | 50 / 200 | View product page |
Positive Control
| Cat. No. | Product Name | Specs | |
|---|---|---|---|
| MD002 | CD-2019-nCoV-abn | 1ml / 5ml / 10ml | View product page |
| MD003 | CD-2019-nCoV-abEN | 1ml / 5ml / 10ml | View product page |
One Step qRT-PCR Kits
| Cat. No. | Product Name | Specs | |
|---|---|---|---|
| QRT001 | CD One Step qRT-PCR SYBR Green Kit | 250 / 100 | View product page |
| QRT002 | CD One Step qRT-PCR Probe Kit | 250 / 100 | View product page |
NGS Metagenome Preparation Kits
| Cat. No. | Product Name | Specs | |
|---|---|---|---|
| DP001 | CD Universal DNA Library Prep Kit for Illumina | 24 / 96 / 24(PCR-free) / 96(PCR-free) | View product page |
| DP002 | CD NEXT DNA Library Prep Kit for Illumina | 24(50ng) / 96(50ng) / 24(5ng) / 96(5ng) / 24(1ng) / 96(1ng) | View product page |
| CB001 | CD DNA Clean Beads | 5ml / 60ml / 450ml | View product page |
| DSI001 | CD DNA Adapters S1-S2 for Illumina | 48 / 192 / 48 / 192 | View product page |
| DSI002 | CD DNA Adapters S3-S6 for Illumina | 192 / 192 / 192 / 192 | View product page |
| DDI001 | CD Multiplex Oligos S1 for Illumina | 192 | View product page |
| DDI002 | CD Multiplex Oligos S2 for Illumina | 192 | View product page |
| DDI003 | CD NEXT Index Kit S1 for Illumina | 192 | View product page |
| DDI004 | CD NEXT Index Kit S2 for Illumina | 768 | View product page |
| DDI005 | CD NEXT Index Kit S3 for Illumina | 192 / 192 / 192 / 192 | View product page |
Please add ethanol (96-100%) to Buffer PW and GD before use, the volume as described on the bottle.>
- Pipet 20 μl Proteinase K into a clean 1.5 ml centrifuge tube (not provided).
- Add 200 μl of plasma/serum/ lymph into the centrifuge tube (Equilibrate the samples to room temperature); or take 140 μl Swab preservation solution or VTM add 0.9% sodium chloride solution to 200 μl.
- Add 200 μl Carrier RNA working solution (mixture of Buffer GB and Carrier RNA solution, please refer to table 1). Close the cap and mix by pulse-vortex for 15 s.
- Incubate at 56°C for 15 min in a heating block. Briefly centrifuge the 1.5 ml tube to remove drops from the inside of the lid.
- Add 250 μl of ethanol (96-100%) to the sample (Precipitates may be visible after addition of ethanol), close the cap and mix thoroughly by pulse-vortex for 15 s. Incubate the lysate with the ethanol for 5 min at room temperature (15-25°C).
- Briefly centrifuge the 1.5 ml centrifuge tube to remove drops from the inside of the lid.
- Carefully transfer the lysate, including any precipitates that may have formed onto the RNase-Free Spin Columns CR2 in a 2 ml RNase-Free Collection Tube without wetting the rim. Close the cap and centrifuge at 8,000 rpm (~6,000 × g) for 1 min. Discard the filtrate; place the spin column in the same collection tube.
- Carefully open the RNase-Free Spin Columns CR2, and add 500 μl of Buffer GD (Ensure that ethanol (96-100%) has been added before use) without wetting the rim. Close the cap and centrifuge at 8,000 rpm (~6,000 × g) for 1 min. Discard the filtrate and place the spin column in the same collection tube.
- Carefully open the RNase-Free Spin Columns CR2, and add 600 μl of Buffer PW (Ensure that ethanol (96-100%) has been added before use) without wetting the rim. Close the cap, let it stand still for 2 min and centrifuge at 8,000 rpm (~6,000 × g) for 1 min. Discard the filtrate and place the spin column in the same collection tube.
- Repeat step 9.
- Carefully open the RNase-Free Spin Column CR2 and add 500 μl of ethanol (96-100%) without wetting the rim. Close the cap and centrifuge at 8,000 rpm (~6,000 × g) for 1 min. Discard the filtrate.
- Place the RNase-Free Spin Column CR2 in the same collection tube. Centrifuge at full speed 12,000 rpm (~13,400 × g) for 3 min to dry the membrane completely.
- Place the RNase-Free Spin Column CR2 in a clean 1.5 ml RNase-Free Centrifuge Tube, carefully open the lid of the spin column, incubate at room temperature for 3 min to dry the membrane completely. Add 20-150 μl of RNase-Free ddH2O to the center of the membrane. Close the lid and incubate at room temperature (15-25°C) for 5 min. Centrifuge at 12,000 rpm (~13,400 × g) for 1 min.
Note: Ensure that the elution buffer is equilibrated to room temperature (15-25°C). If elution is done in small volumes (<50 μl), the elution buffer should be dispensed onto the center of the membrane for complete elution of bound RNA and DNA.
