The CD DNA Adapters S1-S2 for Illumina were created specifically for the CD Universal DNA Library Prep Kit. The kit includes two kits for preparing DNA libraries for Illumina platforms. Universal Adapter as well as 12 different 6bp indexed DNA Adapters that can be used to differentiate between specimens are included in each kit. All Kit components are exposed to a restrictive quality control procedure.
Components:
Application:
Special for DNA library preparation for Illumina platforms with CD Universal DNA Library Prep Kit for Illumina (DP001).
Storage:
All the components can be stored at -20℃ for one year.
Specifications:
| Application | Used for CD Universal DNA Library Prep Kit for Illumina |
| Sequencing Platform | Illumina |
The structure of libraries prepared with CD DNA Adapters S1-S2 for Illumina are as follows:
5' - Universal Adapter - Insert DNA Sequence - DNA Adapter X - 3'
CD Index Primer for Illumina®
Both the Universal Adapter and the Index are contained in each DNA Adapter. The related sequences are as follows:
| Name | Sequence | Index Sequence* |
| Universal Adapter | 5'-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3' | |
| DNA Adapter 1 | 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTG-3' | ATCACG |
| DNA Adapter 2 | 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTG-3' | CGATGT |
| DNA Adapter 3 | 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTG-3' | TTAGGC |
| DNA Adapter 4 | 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTG-3' | TGACCA |
| DNA Adapter 5 | 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTG-3' | ACAGTG |
| DNA Adapter 6 | 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTG-3' | GCCAAT |
| DNA Adapter 7 | 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTG-3' | CAGATC |
| DNA Adapter 8 | 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTG-3' | ACTTGA |
| DNA Adapter 9 | 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTG-3' | GATCAG |
| DNA Adapter 10 | 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTG-3' | TAGCTT |
| DNA Adapter 11 | 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTG-3' | GGCTAC |
| DNA Adapter 12 | 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTG-3' | CTTGTA |
| DNA Adapter 13 | 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTG-3' | AGTCAA |
| DNA Adapter 14 | 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTG-3' | AGTTCC |
| DNA Adapter 15 | 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTG-3' | ATGTCA |
| DNA Adapter 16 | 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTG-3' | CCGTCC |
| DNA Adapter 18 | 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTG-3' | GTCCGC |
| DNA Adapter 19 | 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTG-3' | GTGAAA |
| DNA Adapter 20 | 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTG-3' | GTGGCC |
| DNA Adapter 21 | 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATGCCGTCTTCTGCTTG-3' | GTTTCG |
| DNA Adapter 22 | 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTG-3' | CGTACG |
| DNA Adapter 23 | 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTG-3' | GAGTGG |
| DNA Adapter 25 | 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTG-3' | ACTGAT |
| DNA Adapter 27 | 5'-GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTG-3' | ATTCCT |
* The index sequences (6 bp) are also the index sequences during sequencing, which can be input directly to the Sample Sheet.
Quality Control
16-Hour Incubation: A 50 ul reaction system containing 5 ul of Oligos and 1 μg of Hind III-λ DNA incubated at 37 °C for 16 hours resulted in no band degraded detected by agarose gel electrophoresis. A 50 μl reaction system containing 5 ul of Oligos and 1 μg of T3 DNA incubated at 37 °C for 16 hours resulted in no band degraded detected by agarose gel electrophoresis.
Endonuclease Activity: A 50 ul reaction system containing 5 ul of DNA adapters and 1 ug of pX174RF I DNA incubated at 37°C for 4 hours resulted in < 10% conversion to RF Il analyzed by agarose gel electrophoresis.
