CD Universal DNA Library Prep Kit is compatible with CD Multiplex Oligos S1 for Illumina. Different samples can be distinguished using the dual-indexed adapter. It's designed to make NGS multiplexing more efficient and informative by tracking the sequencing reaction at the molecule level and allowing bioinformatic removal of misassigned reads for maximum accuracy.
Components:
Components of CD Multiplex Oligos S1 for Illumina
Application:
Dedicated adapter primer kit for CD Universal DNA Library Prep Kit for Illumina (DP001), suitable for the generation of double-ended Indexed libraries for Illumina high-throughput sequencing platform.
Storage:
All the components can be stored at -20℃ for one year.
Specifications:
| Application | Used for CD Universal DNA Library Prep Kit for Illumina |
| Sequencing Platform | Illumina |
Adapter-S for Illumina
5´-ACACTCTTTCCCTACACGACGCTCTTCCGATC-s-T-3´ 3´-CTGACCTCAAGTCTGCACACGAGAAGGCTAG-p-5´
(-s- indicates thio, and -p indicates phosphorylation)
i5 PCR Primers
5´-AATGATACGGCGACCACCGAGATCTACAC [i5] АСАСТСТТТСССТАСАСGAСGСTCTTCCGATC-3´
i7 PCR Primers
5´-CAAGCAGAAGACGGCATACGAGAT [i7] GTGACTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´
(-s- indicates thio)
*[i5] represents an 8 nt i5 Index sequence, and [i7] represents an 8 nt i7 Index sequence.
The index name corresponding to each primer, the Index sequence included in the primer, and the Index sequence information input in the Sample Sheet at the time of sequencing are as follows:
Index sequence information for CD Multiplex Oligos S1 for Illumina
