CD Chromatin Immunoprecipitation (ChIP) Assay Kit

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CAT	Specs	Inquiry	Basket
EP006-01	16

Chromatin immunoprecipitation (ChIP) is used to analyze interactions between genomic DNA and histones, transcription factors, or other chromatin-associated proteins. CD Chromatin Immunoprecipitation (ChIP) Assay Kit provides a 16-reaction reference format covering cell fixation, chromatin preparation and fragmentation, antibody-based immunoprecipitation, washing, elution, cross-link reversal, and DNA purification. Reference reaction conditions use 10-30 µg chromatin per immunoprecipitation. Chromatin input, fixation, fragmentation, and antibody amounts should be optimized according to sample type and target abundance. Purified ChIP DNA can be analyzed by qPCR or used for sequencing library preparation. The standard workflow comprises fixation, chromatin preparation, chromatin fragmentation, immunoprecipitation, washing and elution, cross-link reversal, DNA purification, and downstream qPCR or sequencing analysis. For research use only. Not for use in diagnostic procedures.

Storage:

Store each component according to its product label. Avoid repeated freeze/thaw cycles where applicable.

Specifications:

Features	16-reaction reference format. Protein G-based capture and washing workflow. Includes functional reagents and consumables for chromatin preparation, immunoprecipitation, washing, elution, cross-link reversal, and DNA purification. Supports profiling of histone modifications and chromatin-associated proteins. Compatible with ChIP-qPCR and ChIP-seq workflows.
Application	It is recommended to use this kit for: Histone modification profiling Mapping transcription factor binding sites Mapping chromatin-associated protein binding sites ChIP-qPCR ChIP-seq
Species Category	mammalian and other eukaryotic samples
Sample type	Cultured cells or tissues with optimized fixation and chromatin fragmentation conditions
Sample amount	Reference chromatin input: 10-30 µg per immunoprecipitation. Optimize chromatin input, fixation, fragmentation, and antibody amount according to sample type and target abundance.
Sequencing Platform	Illumina; qPCR