The Magnetic Serum/Plasma Circulating DNA Kit is specifically designed for high-throughput circulating DNA isolation from serum/plasma. DNA connects to the silica exterior of the magnetic particles, free of proteins and other contaminants, using magnetic-particle technology and a specific buffer solution. The kit can be used to purify DNA manually or using an automated extraction system. Purified DNA can be utilized directly in molecular biological experiments like PCR, Southern Blot, and next-generation sequencing (NGS). The Kit can be kept at room temperature (15-25°C) for a long time. Serum and plasma are both acceptable sample types. Its processes for isolating circulating DNA from various amounts of plasma/serum ranging from 400 μL to 5 ml are reliable, simple, and high-throughput.
Storage:
Store at room temperature (15-25℃)
Components:
Specifications:
| Sample amount | 0.4-5ml |
| Features | Purified DNA can be used directly for PCR, Southern Blot and NGS. Suitable for DNA purification by manual operation or automated extraction system. Reliable, simple and high-throughput procedures for isolating circulating DNA from various amounts of plasma/serum ranging from 400 μl up to 5 ml. |
| Application | The purified DNA can be used directly for molecular biological experiments such as PCR analysis, Southern Blot and library construction. |
| Sample type | Serum, plasma |
Ensure that ethanol (96-100%) has been added into Buffer RW, isopropanol has been added into Buffer CFL as indicated on the tag of the bottle before use.
- According to the sample volume to choose the centrifuge tube. Pipet the sample into the centrifuge tube. Then add the reagents in turn as follows:
- Mix by pipetting or vortex. Incubate at room temperature for 20 min, and mix by pipetting or vortex for 3-5min. Briefly centrifuge the microcentrifuge tube to remove drops from the inside of the lid.
- Place the centrifuge tube on a magnetic separation device for 2 min. Allow the magnetic particles to completely clear from the solution and then discard the supernatant carefully.
- Remove the centrifuge tube from the magnetic separation device. Add 750 μl Buffer PD, and mix by pipetting or vortex for 30 s, make sure that the magnetic particles are fully resuspended. Briefly centrifuge the microcentrifuge tube to remove drops from the inside of the lid.
- Place the centrifuge tube on a magnetic separation device for 1 min. Allow the magnetic particles to completely clear from the solution and then discard the supernatant carefully.
- Remove the centrifuge tube from the magnetic separation device. Add 750 μl Buffer RW (ensure that ethanol (96-100%) has been added), and mix by pipetting or vortex for 30 s, make sure that the magnetic particles are fully resuspended. Briefly centrifuge the microcentrifuge tube to remove drops from the inside of the lid.
- Place the centrifuge tube on a magnetic separation device for 1 min. Allow the magnetic particles to completely clear from the solution and then discard the supernatant carefully.
- Repeat step 6 and 7.
- Place the centrifuge tube on the magnetic separation device for 5-10 min.
- Remove the centrifuge tube from magnetic separation device. Add 30-65 μl Buffer TBC to elute nucleic acids. Mix by pipetting or vortex to resuspend the magnetic particles. Incubate at 56°C for 5 min, vortex every 2 min.
- Place the centrifuge tube on the magnetic separation device for 2 min until all the magnetic particles are cleared from the solution. Transfer the supernatant containing purified nucleic acids to a new centrifuge tube and stores it.
| Sample Volume | Centrifuge Tube Specification |
Buffer CFL | Proteinase K | MagAttract Suspension G |
| 400μl | 1.5 ml | 600μl | 40μl | 30μl |
| 600μl | 900μl | 60μl | ||
| 2000μl | 5 ml | 3000μl | 200μl | 45μl |
| 4000μl | 15 ml | 6000μl | 400μl | 90μl |
