CD Magnetic Buccal Swab/Saliva DNA Kit

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DCE103-01 50
DCE103-02 200

CD Magnetic Buccal Swab/Saliva DNA Kit is specially designed for genomic DNA isolation from buccal swab, saliva, mouthwash and other buccal sample types fast and high-throughput. Based on magnetic-particle technology and specific buffer solution, DNA binds to the silica surface of the magnetic particles free from proteins and other contaminants. The kit is suitable for DNA purification by manual operation or automated extraction system. The purified DNA can be used directly for molecular biological experiments such as restriction analysis, PCR analysis, Southern Blot and library construction.

The Magnetic Buccal Swab/Saliva DNA Kit is specifically designed for rapid and high-throughput genomic DNA isolation from buccal swabs, saliva, mouthwash, and other buccal specimen types. DNA attaches to the silica surface of the magnetic particles, free of proteins and other contaminants, using magnetic-particle innovation and a specific buffer solution. The kit can be used to purify DNA manually or with an automated extraction system. Restriction analysis, PCR analysis, Southern Blot, and library construction can all be done directly with purified DNA. The Kit can be kept at room temperature (15-25°C) for a long time. Buccal swab, saliva, mouthwash, and other buccal sample types are all acceptable.

Storage:

Store at room temperature (15-25℃)

Components:

CD Magnetic Buccal Swab/Saliva DNA Kit-Components

Specifications:

Features Purified genomic DNA can be extracted from buccal swab, saliva, mouthwash and other buccal sample types within one hour. Purified DNA can be used directly for PCR, restriction digestion and Southern Blot. Suitable for DNA purification by manual operation or automated extraction system.
Application The purified DNA can be used directly for molecular biological experiments such as restriction analysis, PCR analysis, Southern Blot and library construction.
Sample type Buccal swab, saliva, mouthwash and other buccal sample types

Ensure that ethanol (96-100%) has been added into Buffer PWD, isopropanol has been added into Buffer GHC as indicated on the tag of the bottle before use.

  1. Sample preparation:
    a. For Buccal swab samples: To collect buccal cells, scrape the inside of the buccal cavity 10 times with a buccal swab. Dispense 500 μl Buffer GHA into a 2 ml microcentrifuge tube. Remove the collection swab from its handle using sterile scissors, and place the detached head in the tube. Add 10μl Proteinase K, close the lid, and mix by pulse-vortex for 10 s. Incubate at 65°C for 15-30 min. Vortex every 10 min. Pipet 300μl into a new microcentrifuge tube used for the next step.
    b. For pharyngeal swab samples: To collect pharyngeal cells, scrape the inside of the pharyngeal 10 times with a pharyngeal swab. Dispense 1-2ml Buffer GHA into a 5 ml microcentrifuge tube. Remove the collection swab from its handle using sterile scissors, and place the detached head in the tube, mix thoroughly by upsides and down. Pipet 300μl into a new microcentrifuge tube, then add 10μl Proteinase K, close the lid, and mix by pulse-vortex for 10 s. Incubate at 65°C for 30 min. Vortex every 10 min.
    c. For fresh saliva samples: Prior to collection of saliva samples, the donor should rinse their mouth with a few milliliters of water for 10 seconds in order to remove any food particles that may be present. Ten minutes after rinsing, collect saliva by spitting into a sterile collection tube or vial (not supplied).  The amount of saliva collected should be at least 100μl but not more than 2 ml. Add equal volume of Buffer GHA, mix thoroughly by upsides and down. Pipet 300μl into a new microcentrifuge tube, then add 10μl Proteinase K, close the lid, and mix by pulse-vortex for 10 s. Incubate at 65°C for 30 min. Vortex every 10 min.
  2. Add 600μl Buffer GHC (Ensure isopropanol has been added), and mix by pipetting or vortex.
  3. Add 10μl MagAttract Suspension G, mix by pipetting or vortex for 12min.
  4. Place the centrifuge tube on a magnetic separation device for 30 s.  Allow the magnetic particles to completely clear from the solution and then discard the supernatant carefully.
  5. Remove the centrifuge tube from the magnetic separation device. Add 900 μl Buffer PD, and mix by pipetting or vortex for 3min.
  6. Place the centrifuge tube on a magnetic separation device for 30 s.  Allow the magnetic particles to completely clear from the solution and then discard the supernatant carefully.
  7. Remove the centrifuge tube from the magnetic separation device. Add 900 μl Buffer PD, and mix by pipetting or vortex for 3min.
  8. Place the centrifuge tube on a magnetic separation device for 30 s.  Allow the magnetic particles to completely clear from the solution and then discard the supernatant carefully.
  9. Remove the centrifuge tube from the magnetic separation device. Add 900 μl Buffer PWD (ensure that ethanol (96-100%) has been added), and mix by pipetting or vortex for 3min.
  10. Place the centrifuge tube on a magnetic separation device for 30 s.  Allow the magnetic particles to completely clear from the solution and then discard the supernatant carefully.
  11. Place the centrifuge tube on the magnetic separation device for 10-15 min
  12. Remove the centrifuge tube from magnetic separation device. Add 50-100 μl Buffer TB to elute nucleic acids. Mix by pipetting or vortex and incubate at 56°C for 10 min, vortex every 3 min.
  13. Place the centrifuge tube on the magnetic separation device for 2 min until all the magnetic particles are cleared from the solution. Transfer the supernatant containing purified nucleic acids to a new centrifuge tube and stores it.

* For Research Use Only. Not for use in diagnostic procedures.

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