CD RNA Adapters S1-S2 for Illumina are designed specifically for the preparation of CD RNA libraries on Illumina platforms. It's a distinct kit for Illumina high-throughput sequencing platform library construction that's appropriate for multi-specimen RNA library construction on the Illumina high-throughput sequencing platform. Two kits are included in the kit. Each kit includes a Universal Adapter as well as 12 different 6bp indexed RNA Adapters that can be used to differentiate between samples.
Components:
Application:
Special for RNA library preparation for Illumina platforms with CD mRNA-seq Library Prep Kit for Illumina (RP001), CD Stranded mRNA-seq Library Prep Kit for Illumina (RP002) or CD Total RNA-seq Library Prep Kit for Illumina(human/mouse/rat) (RP003).
Storage:
All the components can be stored at -20℃ for one year.
Specifications:
| Application | Used for: CD mRNA-seq Library Prep Kit for Illumina CD Stranded mRNA-seq Library Prep Kit for Illumina CD Total RNA-seq Library Prep Kit for Illumina(human/mouse/rat) |
| Sequencing Platform | Illumina |
The structure of libraries prepared with CD RNA Adapters s1-s2 for Illumina are as follows:
5’ - Universal Adapter - Insert RNA Sequence - DNA Adapter X - 3’
CD Index Primer for Illumina®
Both the Universal Adapter and the Index are contained in each DNA Adapter. The related sequences are as follows:
| Name | Sequence | Index Sequence* |
| Universal Adapter | 5’-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3’ | |
| RNA Adapter 1 | 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTG-3’ | ATCACG |
| RNA Adapter 2 | 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTG-3’ | CGATGT |
| RNA Adapter 3 | 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTG-3’ | TTAGGC |
| RNA Adapter 4 | 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTG-3’ | TGACCA |
| RNA Adapter 5 | 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTG-3’ | ACAGTG |
| RNA Adapter 6 | 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTG-3’ | GCCAAT |
| RNA Adapter 7 | 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTG-3’ | CAGATC |
| RNA Adapter 8 | 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTG-3’ | ACTTGA |
| RNA Adapter 9 | 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTG-3’ | GATCAG |
| RNA Adapter 10 | 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTG-3’ | TAGCTT |
| RNA Adapter 11 | 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTG-3’ | GGCTAC |
| RNA Adapter 12 | 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTG-3’ | CTTGTA |
| RNA Adapter 13 | 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTG-3’ | AGTCAA |
| RNA Adapter 14 | 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTG-3’ | AGTTCC |
| RNA Adapter 15 | 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTG-3’ | ATGTCA |
| RNA Adapter 16 | 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTG-3’ | CCGTCC |
| RNA Adapter 18 | 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTG-3’ | GTCCGC |
| RNA Adapter 19 | 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTG-3’ | GTGAAA |
| RNA Adapter 20 | 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTG-3’ | GTGGCC |
| RNA Adapter 21 | 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATGCCGTCTTCTGCTTG-3’ | GTTTCG |
| RNA Adapter 22 | 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTG-3’ | CGTACG |
| RNA Adapter 23 | 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTG-3’ | GAGTGG |
| RNA Adapter 25 | 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTG-3’ | ACTGAT |
| RNA Adapter 27 | 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTG-3’ | ATTCCT |
* The index sequences (6 bp) are also the index sequences during sequencing, which can be input directly to the Sample Sheet.
Quality Control
16-Hour Incubation: A 50 ul reaction system containing 5 ul of Oligos and 1 μg of Hind III-λ DNA incubated at 37 °C for 16 hours resulted in no band degraded detected by agarose gel electrophoresis. A 50 μl reaction system containing 5 ul of Oligos and 1 μg of T3 DNA incubated at 37 °C for 16 hours resulted in no band degraded detected by agarose gel electrophoresis.
Endonuclease Activity: A 50 ul reaction system containing 5 ul of RNA adapters and 1 ug of pX174RF I DNA incubated at 37°C for 4 hours resulted in < 10% conversion to RF Il analyzed by agarose gel electrophoresis.
