The RNA Clean Kit is specifically designed for total RNA cleanup and concentration from enzymatic reaction products (DNase treated, Protease treated, RNA marked) and other specimens in a fast and simple technique. The maximum sample size that this kit can handle is 20g. Its quick and easy workflow allows for total RNA cleanup and concentration in under 30 minutes. Proteins, salts, and organic compounds are removed using unique silica-membrane technology and a special buffer solution. Total RNA that has been purified can be used directly in molecular biological experiments like Northern Blots, cDNA synthesis, and primer extension, among others. It's worth noting that the Kit can be kept at room temperature (15-25°C).
Storage:
Store at room temperature (15-25℃)
Components:
Specifications:
| Features | Fast and convenient workflow enables total RNA cleanup and concentration within 30min. |
| Application | Total RNA cleanup and concentration from products of enzymatic reactions (DNase treated, Protease treated, RNA marked) and other samples. The purified total RNA can be used directly for molecular biological experiments such as Northern Blot, cDNA synthesis, and primer extension, etc. |
| Sample type | total RNA from products of enzymatic reactions (DNase treated, Protease treated, RNA marked) and other samples |
- Add RNase-Free ddH2O in RNA sample up to 100l, add 350l Buffer RK (Ensure that β-ME has been added). Vortex vigorously.
- Add 250 µl ethanol (96% -100%), vortex vigorously.
- Transfer the mixture and precipitate to a RNase-Free Columns CR2 set (put CR2 into collection tube) immediately, centrifuge for 30s at 12,000 rpm (~13,400 × g), discard the flow-through in the collection tube, and put the spin column back to the collection tube.
- Add 500 µl Buffer RW (Ensure that ethanol (96–100%) has been added), stand for 2 min at room temperature (15-25°C), centrifuge for 30s at 12,000 rpm (~13,400 × g), discard the flow-through in the collection tube, and put the spin column back to the collection tube.
- Repeat step 4.
- Centrifuge for 5min at 12,000 rpm (~13,400 × g), discard the flow-through in the collection tube.
- Discard the Collection Tube and transfer the Spin Column CB2 to a clean 1.5 ml centrifuge tube, add 14-20 µl RNase-Free ddH2O, stand for 2 min at room temperature (15-25°C), centrifuge for 2min at 12,000 rpm (~13,400 × g).
- Repeat step 7 to increase RNA recovery efficiency.
If the volume of eluted buffer is less than 14 μl, it may affect recovery efficiency.
The pH value of eluted buffer will have big influence in eluting; distilled water (pH 7.0-8.5, adjusted with NaOH) is suggested to elute RNA, pH<7.0 will decrease elution efficiency. For long-term storage of RNA, storing at -20°C is recommended.
