The Magnetic Animal Genomic DNA Kit is specifically designed for fast and high-throughput genomic DNA isolation from a variety of tissues, including whole blood samples. Its simple and quick workflow allows for DNA isolation in under an hour. There is no need for phenol-chloroform or other hazardous solvents. Based on magnetic-particle technology and specific buffer solution, DNA binds to the silica surface of the magnetic particles free from proteins and other contaminants. The kit is suitable for DNA purification by manual operation or an automated extraction system. The purified DNA can be used directly for molecular biological experiments such as PCR, qPCR, restriction enzyme digestion, Southern hybridization, and DNA library construction.
Storage:
Store at room temperature (15-25℃)
Components:
Specifications:
| Features | Fast and convenient workflow enables DNA isolation within 1h. Neither phenol-chloroform nor other hazardous solvents are needed. The kit is suitable for DNA purification by manual operation or automated extraction system, such as Thermo, Eppendorf, Tecan, Hamilton, and Beckman, etc. |
| Application | The purified DNA can be used directly for molecular biological experiments such as PCR, qPCR, restriction enzyme digestion, Southern hybridization and DNA library construction. |
| Sample type | Suits to different kinds of tissues, especially whole blood samples. |
Ensure that Ethanol (96-100%) has been added into Buffer GD and Buffer PW before use as indicated on the tag.
- Add 20 μl of Proteinase K into microcentrifuge tube or 96 deep well plate.
Note: For more samples, premix first. The mixture of proportion is 20 μl proteinase K per 200 μl Buffer GB. - Add sample:
a. Whole blood: Transfer 100 μl whole blood (transfer the stored blood after completely thawed) to micro-centrifuge tube or 96 deep well plate.
b. Dried blood spots: Three pieces of 3 x 3 mm
c. Tissues: 10 mg animal tissue. - Add 200 μl Buffer GB, and mix completely by pipetting or vortex.
- Sample digestion
a. Blood sample: Incubate at 56°C for 10 min
b. Dried blood spots: Incubate at 56°C for 10 min
c. Tissues: Incubate at 56°C until the tissue is completely digested.
For magnetic beads fully suspended, please vortex before use. - Add 200 μl ethanol (96-100%) to each well and mix thoroughly by pipetting or vortex. Place the sample on the bench for 5 min at room temperature.
- Add 15 μl MagAttract Suspension B for each well, and mix by pipetting or vortex.
- Place 96 deep well plate on a magnetic separation device for 30 s. Allow the magnetic particles to completely clear from the solution and then discard the supernatant carefully.
- Remove the 96 deep well plate from the magnetic separation device. Add 500 μl Buffer GD (ensure that ethanol (96-100%) has been added into Buffer GD before use), and mix by pipetting or vortex.
- Place the 96 deep well plate onto the magnetic separation device for 30 s. Allow the magnetic particles to completely clear from the solution and then discard the supernatant carefully.
- Remove the 96 deep well plate containing magnetic Particles from the magnetic separation device. Add 600 μl Buffer PW (Ensure that Ethanol (96-100%) has been added into Buffer PW before use), and mix by vortex or pipetting.
- Place the 96 deep well plate onto the magnetic separation device for 30 s. Allow the magnetic particles to completely clear from the solution and then discard the supernatant carefully.
- Repeat step 10 and 11, clear the solution as possible.
- Place 96 deep well plate on the magnetic separation device for 10-15 min to air dry.
- Remove the plate from magnetic separation device. Add 50-100 μl Buffer TB to elute DNA. Mix by pipetting or vortex and incubate at 56°C for 10 min.
- Place the plate on the magnetic separation device for 30 s until all the magnetic particles are cleared from the solution. Transfer the supernatant containing purified DNA to a new collection plate and stores it.
