CD FFPE DNA Kit

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DCE005-01 50

CD FFPE DNA Kit is specially designed for genomic DNA isolation from formalin-fixed, paraffin-embedded tissues, suitable for a wide range of samples. The Kit uses unique deparaffinization solution and specific lysis condition to release DNA which overcomes the inhibitory effect of formalin. Based on the unique silica membrane technology and its special buffer solution, the Kit provides high-purity and high-integrity DNA into a small elution volume. The purified DNA can be used directly for molecular biological experiments such as PCR, restriction enzyme digestion and Southern hybridization.

The FFPE DNA Kit was created specifically for isolating genomic DNA from formalin-fixed, paraffin-embedded tissues and is appropriate for a variety of samples. The Kit overpowers the inhibitory effect of formalin by using a unique deparaffinization solution and a specific lysis condition to release DNA. Furthermore, the one-of-a-kind deparaffinization solution is safer than xylene. The Kit delivers high-purity and high-integrity DNA in a small elution volume thanks to its unique silica-membrane technology and special buffer solution. Purified DNA can be used right away in molecular biology experiments like PCR, restriction enzyme digestion, and Southern hybridization. Roughly 5-8 pieces of 5-10 m of the sample are required.

Storage:

Store at room temperature (15-25℃)

Components:

CD FFPE DNA Kit-Components

Specifications:

Sample amount 5-8 pieces, 5-10 μm
Features Fast and convenient workflow enables DNA isolation within 1h. The unique deparaffinization solution is more safety than xylene.
Application The purified DNA can be used directly for molecular biological experiments such as PCR, restriction enzyme digestion and Southern hybridization.
Sample type FFPE tissues

Ensure that ethanol (96-100%) has been added into Buffer GD and Buffer PW as indicated on the tag of the bottle before use.

  1. Sample preparation:
    a. Paraffin section: take 5-8 paraffin sections (each section with thickness of 5 - 10 μm and surface area of 1 × 1 cm2).
    b. Paraffin Block: remove excess paraffin off the sample by a scalpel, cut up the tissue sample (30 mg) into as small pieces as possible.
    Note: If the sample surface has been exposed to air, discard the first 2-3 sections.
    c. Samples of formalin and other fixative solutions: cut up the tissue sample (30 mg) into as small pieces as possible by a scalpel, and transfer into a 1.5 ml centrifuge tube, add 500μl PBS (10mM, pH7.4), centrifuge for 1min at 12,000 rpm (~13,400 ×g), and discard the flow-through, repeat 3 times.
  2. Immediately place the sample in a 1.5 ml centrifuge tube. Add 500μl Buffer GL, 50μl Buffer GP, vortex vigorously for 10 s.
  3. Incubated at 98 °C for 30 min, mix the tube for several times during incubation, until the sample is completely dissolved.
  4. Centrifuge for 5 min at 12,000 rpm (~13,400 × g).
  5. Transfer the clear aqueous phase of the mesosphere into a new centrifuge tube.
  6. (optional) Add 2μl RNase A(100mg/ml), incubated for 2 min at room temperature (15-25°C).
  7. Add 2 volume of Ethanol (96%-100%) (For example, add 900 μl Ethanol (96%-100%) to 450 μl aqueous phase) to mix, stand for 3 min at room temperature (15-25°C).
  8. Transfer the mixture to Spin Columns CR2 (put CR2 into collection tube), centrifuge for 2min at 8,000 rpm (~6,000 ×g). Discard the flow-through in the collection tube, and put the spin column back to the collection tube.
  9. Add 500μl Buffer GD (Ensure that ethanol (96-100%) has been added) to Spin Columns CR2, centrifuge for 1min at 8,000 rpm (~6,000 ×g). Discard the flow-through in the collection tube, and put the spin column back to the collection tube.
  10. Add 600μl Buffer PW (Ensure that ethanol (96-100%) has been added) to Spin Columns CR2, centrifuge for 1min at 8,000 rpm (~6,000 ×g). Discard the flow-through in the collection tube, and put the spin column back to the collection tube.
  11. Repeat step 10.
  12. Put the spin column back to the collection tube, centrifuge for 2 min at 12,000 rpm (~13,400 × g), discard the flow-through in the collection tube. Open the lid and place the Spin Column CB2 at room temperature (15-25°C) for several minutes to dry membrane completely.
  13. Discard the Collection Tube and transfer the Spin Column CB2 to a clean 1.5 ml centrifuge tube. Pipet 30-100 μl 65°C preheated Buffer TE or ddH2O directly onto the Spin Column CB2 membrane, incubate for 2-5 min at room temperature (15-25°C), and then centrifuge for 2 min at 12,000 rpm
    (~13,400 × g) to elute.

* For Research Use Only. Not for use in diagnostic procedures.

We provide high-quality kit products for researchers from across the world, meeting the needs of various nucleic acid-related experiments.

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