CDAMP® Lung Cancer 23 Panel Kit


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CDAMP® Lung Cancer 23 Panel Kit: pdf
CDAMP® Lung Cancer 23 Panel Kit Gene List: pdf
Product Infomation
Product Description: CDAMP® Lung Cancer 23 Panel Kit is specially designed for target enrichment with unique molecular identifiers(UMIs) labeled and library preparation using a fast and simple workflow, in next generation sequencing (NGS) applications on Illumina platforms. The panel contains 53 amplicons covered a total of 23 hotspot genes associated with lung cancer. The Kit enables library preparation from as low as 20ng DNA and allows a single library construction within 3.5 hours. Based on UMI technology, which is a multiplex PCR reaction that uses UMI-labeled target-specific primers to barcode and amplify targets of interest, the panel enable bioinformatically correction of amplification and sequencing errors, so that low-frequency variants below 0.5% allele frequency can be detected with just 30 ng of input DNA. This kit provides all required enzymes and buffers for Multiplex PCR Reaction, Purification, Indexing and Amplification. Magnetic beads can be used in library purification rapidly. All Kit components are subjected to stringent quality control ensuring the consistency and reproducibility of library preparation.
Features: Ready-to-use and containing all necessary reagents for a rapid set-up. Fast and streamlined workflow enables library preparation in just 3.5 hours. Low-frequency variants below 0.5% allele frequency can be detected with just 30 ng of input DNA. Optimized to deliver high specificity (≥95%) and high coverage uniformity (≥95%) sequencing results. All Kit components are subjected to stringent quality control ensuring the consistency and reproducibility of library preparation.
Enrichment Method: Amplicon sequencing
Number of Genes: 23
Target Size: 1.9kb
Amplicon Length : 70-166bp
Number of Primer Pools: 1
Sample Input Size : ≥30ng per pool for high quality gDNA
Species: human
Sample Type: Genomic DNA from tissues, formalin-fixed paraffin-embedded (FFPE) tissues; Cell-free DNA (cfDNA)
Sequencing Platform: Illumina
Application: It is recommended to use this kit for: Variant detection (Single nucleotide variations, insertions-deletions) Low-frequency variants below 0.5% allele frequency
Storage: All the components should be stored at -20℃.
* For Research Use Only. Not for use in diagnostic procedures.

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