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BAC & Fosmid Sequencing

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The Introduction of BAC & Fosmid Sequencing

BACs (Bacterial Artificial Chromosomes) are the most popular genomic DNA construct and have been used extensively for genome characterization and in hierarchical sequencing projects like the human genome. BAC libraries offer a source of large genomic inserts (up to 200 Kbp) that facilitate studies of genomic content without a reference sequence. In addition to the BAC resource, fosmid sequences have been also used in genomic studies. Fosmids are less expensive to generate than BACs and have less cloning bias; their insert size (around 35–40 kb) can also be more narrowly controlled. BAC & Fosmid are an ideal resource for preliminary genome characterization and for improving whole-genome shotgun assemblies, and can detect structural variation such as insertions, deletions, and inversions.

Sanger sequencing has some problems, so that conventional BAC & Fosmid libraries are inherently biased and incomplete. Therefore, massively parallel genome-sequencing technologies no longer rely on cloning DNA fragments in a bacterial host. We use next-generation sequencing technology, sequencing BAC & Fosmid clones can simplify the assembly of a complex genome at the highest quality, and paired-end sequencing of large DNA fragments cloned in BAC & Fosmid vectors are a mainstay of genome projects and essential tools for genome research.

  • Reconstruct medically and developmentally important structures within complex regions
  • Finely map genes of interest with structural variation and haplotype information for evolutionary and agricultural studies
  • Generate platinum-level genome assemblies
  • Resolve historically difficult-to-sequence regions in draft genomes

Key Features and Advantages

  • Unbiased BAC & Fosmid libraries without gaps,
  • Guaranteed coverage for each library,
  • Efficient and cost-effective sequencing, especially suitable for large-scale BAC & Fosmid sequencing.

Project Workflow

BAC & Fosmid Sequencing Workflow

Data Analysis

  • Data quality control
  • Clean Data assembly
  • Assembly result analysis
  • Comparative genomic analysis
  • More data mining upon your request
Sample Requirements

DNA amount: ≥ 1 μg; DNA concentration ≥ 100 ng/μl,

Sequencing Strategy

  • Illumina Hiseq PE150;
  • The average read depth more than 50x coverage

CD Genomics provides full BAC & Fosmid sequencing service package including sample preparation, library construction, Hiseq sequencing, and downstream bioinformatics analysis. Our team of sequencing specialists has extensive NGS experience with handling many sample types and applications. And we can tailor this pipeline to your research interest. If you have additional requirements or questions, please feel free to contact us, our specialists are more than happy to assist you.

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