Hiseq 2500 Sequencing

Sequencing with speed and quality is never before possible.

HiSeq 2500 Sequencing

The HiSeq 2500 system is the latest addition to the revolutionary HiSeq family of sequencers. HiSeq 2500 can be switched between a high output run mode and rapid turnaround run mode. In high output mode, the HiSeq 2500 enables sequencing of a large number of genomes, exomes, or transcriptomes in a single run, allowing large studies to be completed with the fewest number of runs. In rapid run mode, the HiSeq 2500 is capable of sequencing a 30×genome in a day or completing or fast multiplexed applications such as exome resequencing or RNA-Seq. The rapid run time includes automated, on-instrument cluster generation, and paired-end sequencing, with no user intervention needed throughout the entire process.

Highlights:

Unprecedented Speed and Flexibility
Generate 120 Gb in 27 hours in rapid run mode, or 600Gb in high output mode.
Unsurpassed Data Quality
Proven SBS chemistry provides the most accurate
results for potential clinical utility.
Broadest Applications
Run any application for a wide range of study sizes,
All within your budget.

HiSeq 2500 Preliminary Performance Parameters
Performance Parameter	High Output Mode	Rapid Run Mode
Read length (bp)	2 × 100	2 × 100	2 × 150
Yield (Gb)	~600	~120	~180
Run time	~11 days	~27 hours	~39 hours
Bases > Q30	> 80%	> 80%	> 75%
Reads passing filter	> 90%	> 90%	> 90%
Number of flow cells	2	2	2
Lanes/flow cell	8	2	2
Cluster generation	cBot	onboard	onboard