Sequencing with speed and quality is never before possible.
The HiSeq 2500 system is the latest addition to the revolutionary HiSeq family of sequencers. HiSeq 2500 can be switched between a high output run mode and rapid turnaround run mode. In high output mode, the HiSeq 2500 enables sequencing of a large number of genomes, exomes, or transcriptomes in a single run, allowing large studies to be completed with the fewest number of runs. In rapid run mode, the HiSeq 2500 is capable of sequencing a 30×genome in a day or completing or fast multiplexed applications such as exome resequencing or RNA-Seq. The rapid run time includes automated, on-instrument cluster generation, and paired-end sequencing, with no user intervention needed throughout the entire process.
Highlights:
HiSeq 2500 Preliminary Performance Parameters |
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Performance Parameter |
High Output Mode |
Rapid Run Mode |
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Read length (bp) |
2 × 100 |
2 × 100 |
2 × 150 |
|
Yield (Gb) |
~600 |
~120 |
~180 |
|
Run time |
~11 days |
~27 hours |
~39 hours |
|
Bases > Q30 |
> 80% |
> 80% |
> 75% |
|
Reads passing filter |
> 90% |
> 90% |
> 90% |
|
Number of flow cells |
2 |
2 |
2 |
|
Lanes/flow cell |
8 |
2 |
2 |
|
Cluster generation |
cBot |
onboard |
onboard |