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- Sequencing Library Prep Kit
GenSeq™ Post-Bisulfite DNA Sequencing Kit
- Cat. No.
- Kit Size
- 24 actions
- GenSeq™ Post-Bisulfite DNA Sequencing Kit is a complete set of optimized reagents to prepare a DNA library -- after successful bisulfite conversion -- for various Illumina platform-based bisulfite sequencing (bisulfite-seq) assays, such as whole genome bisulfite sequencing (WGBS), oxidative bisulfite sequencing (oxBs-seq), reduced representation bisulfite sequencing (RRBS), and other bisulfite-based next generation sequencing applications. The optimized protocol and components of the kit allow both non-barcoded (singleplexed) and barcoded(multiplexed) DNA libraries to be quickly constructed using sub-nanogram input concentrations of DNA since the DNA is first bisulfite-converted and then used for library preparation.
- Post-Bisulfite DNA Sequencing
- Sample Type
- Bisulfite-treated DNA
- Store at -20°C
- Dry Ice
- For research use only.
- Platform suitable
- Input amount
- 1 ng to 1 µg
- Allows bisulfite-converted DNA to be used directly for ligation, thereby eliminating the possibility of breaking adapter-ligated fragments, which can often occur in currently used WGBS and RRBS methods.
Gel-free size selection/purification saves time and prevents handling errors, as well as loss of valuable samples.
High sensitivty and efficiency.
Ultra HiFi amplification enables achievement of reproducibly high yields of DNA libraries with minimal sequence bias and low error rates.