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GenSeq™ Metagenomic DNA Isolation Kit for Water
- Cat. No.
- Kit Size
- 20 actions
- GenSeq™ Metagenomic DNA Isolation Kit for Water was developed to isolate metagenomic DNA directly from culturable or unculturable microbes in a water sample, to generate cloning-ready DNA for construction of random-sheared fosmid libraries. Traditional methods for isolation of metagenomic DNA to be used in fosmid library construction involve agarose plugs, random shearing, and a size selection using pulse-field gel electrophoresis before cloning into a fosmid vector. These methods are time-consuming, inefficient, and result in a library with biased genome representation. The Metagenomic DNA Isolation Kit for Water isolates high-molecular-weight DNA that is randomly sheared (with fragment sizes approximately 40 kb), does not require size selection, and can be used directly for end-repair and cloning.
- Cloning, PCR or Next Generation Sequencing
- Store at -20°C
- Dry Ice
- For research use only.
- Easy isolation of fosmid cloning-ready metagenomic DNA from microbes, including Gram-positive bacteria, in a water sample.
Isolation of metagenomic DNA for PCR or next-gen sequencing.