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Home >
Custom libraries
> Custom genomic DNA library construction |
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Custom genomic DNA library construction
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A whole genome shotgun
library can be utilized efficiently for
generating DNA sequences or identifying specific
genes from total genomic DNA. CD Genomics
offers highly flexible genomic shotgun library
construction services. We have extensive
experience in constructing shotgun libraries of
high quality from a variety of sources including
bacterial, phage, Mammalian and BAC DNA. |
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Construction:
- High quality DNA
template preparation
- Random shearing to
produce a population of
DNA fragments
- Ligation into a
standard cloning vector
- Transformation &
glycerol stock
production
- QA/QC by DNA
sequencing
- gDNA preparation
available
- 3~4 weeks turnaround
time in most cases
- Final report
including original
electropherograms and
one plate (96 wells)
sequencing result
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Properties:
- Customized insert
sizes: typically <10kb
- Primary Clones: 2x107
(small insert), 1x107
(large insert)
- Percentage of
Recombinants: >95%
(small insert), >90%
(large insert)
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