| |
Home >
Custom libraries
> Custom cDNA Library Construction |
|
| |
|
|
|
Custom cDNA Library Construction
|
|
| |
cDNA library construction is a key step in gene
discovery through EST sequencing or analysis of
full-length cDNA. Using premium protocols, CD
Genomics provides highly quality services of
custom cDNA library construction including
standard cDNA library, large insert cDNA library
and normalized cDNA library. The library
construction service is of particular importance
for scientists who prefer fast access to a high
quality cDNA source of interest. Our technical
staff will coordinate with clients to optimize
the service package to meet specific project
goals and manage the projects from beginning to
end. |
|
| |
|
|
| |
Features:
|
|
| |
- Average cDNA insert length >1kb
- Primary clones >1 x 106
- Recombinants for plasmid library
>90%
- Antibiotic resistance:
Chloromycetin = 25ug/ml
- One plate (96-well) sequencing
for free
- <10% redundancy after
normalization
|
|
| |
Service includes:
|
|
| |
- Sample quality control
- 1st strand synthesis
- Adapter ligation
- Second strand synthesis and
amplification
- Size selection
- Ligation into pDNR-Lib
- Transformation of ligation into
E. coli DH10B
- PCR amplification of the inserts
from 24 randomly picked clones to
determine a percentage of
recombinant clones and average
insert size
|
|
| |
|
|
| |
Deliverables:
|
|
| |
- Primary library stored in -20℃
- Library evaluation report,
including No. of library clones,
average insert size, recombinant
rates, and integrality of cDNA, etc.
- Electrophoresis profiles
|
|
| |
|
|
| |
|
|
| |
|
|
| |
|
|
