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45-1 Ramsey Road, Shirley,
NY 11967, USA
Tel: 1-631-372-1052
Fax: 1-631-614-7828
Email:

Europe
Tel: 44-207-048-3343

cDNA Library Construction Service


cDNA library construction service

cDNA Library Construction Service

CD Genomics has extensive experience in constructing high quality cDNA libraries in a vector of choice. The cDNA libraries are cloned into a standard high-copy vector, picked and arrayed into either 96-well or 384-well microtitre plates amenable to most high throughput DNA sequencing production systems. The libraries are well-suited for EST sequencing and other gene discovery applications. CD Genomics can also sequence ESTs from cDNA libraries via Sanger sequencing or 454 sequencing.


The steps of cDNA library constructionThe Steps of cDNA Library Construction

  • Extraction of RNA from a variety of samples
  • Generation of phage-or plasmid-based cDNA libraries from size-selected cDNA
  • Directional cloning of cDNAs into any vector
  • Production of a library with at least 106  cfu
  • Library amplification to generate higher titer library
  • Library delivered on plates, filters or glycerol stock
  • Verification of library quality by randomly picking clones and determining insert sizes by restriction digest
  • Verification of library complexity by DNA sequencing of the inserts from a subset of randomly picked clones (Optional)






Our cDNA library  construction serviceList of Our cDNA Library Construction Services:


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CONTACT CD GENOMICS
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-372-1052
Fax: 1-631-614-7828
Email:

Europe
Tel: 44-207-048-3343

SERVICES
Sequencing
Microarray
Genotyping
Aptamers
Custom Libraries
Technology Platforms
Products

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