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45-1 Ramsey Road, Shirley,
NY 11967, USA
Tel: 1-631-372-1052
Fax: 1-631-614-7828
Email:

Europe
Tel: 44-207-048-3343

Yeast Two-hybrid cDNA Library Construction Service


Yeast Two-hybrid cDNA Library Construction service

Yeast Two-hybrid cDNA Library

The establishment of the yeast two-hybrid library is based on the understanding of the regulation of the transcription initiation process of eukaryotic cells. Many eukaryotic transcription activation factors come from two separate, functionally independent domains. However, a separate DNA binding domain cannot activate gene transcription, and a separate transcription activation domain cannot activate the UAS downstream genes. The DNA binding domain and transcription activation domain have to be combined to derive the complete function of the transcription activation factor.
The yeast two-hybrid library can be used to study the interaction between known proteins, to find the domain that plays a key role in protein-protein interaction and to discover a new protein interaction with target proteins.

 

The Yeast Two-hybrid Library Has the Following Applications:

  •   1) Interactions between new proteins and protein identification;
  •   2) Protein cascade substrate identification;
  •   3) Effects of mutations on protein and protein binding identification;
  •   4) Identification of interfered protein among known interactions.

Workflow of Yeast Two-hybrid Library Construction:

Workflow of Yeast Two-hybrid Library Construction

We are capable of constructing either full length yeast two-hybrid cDNA library or normalized yeast two-hybrid cDNA library in accordance with your request, We can even build yeast one-hybrid cDNA library and yeast three-hybrid cDNA library at you service.


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CONTACT CD GENOMICS
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-372-1052
Fax: 1-631-614-7828
Email:

Europe
Tel: 44-207-048-3343

SERVICES
Sequencing
Microarray
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Custom Libraries
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