SSH cDNA Library
Suppression subtractive hybridization (SSH) is one of the most powerful and popular PCR-based methods for isolating differentially expressed transcripts. Using a small quantity of either total RNA or poly(A)+ mRNA from each of two populations, the SSH procedure simultaneously subtracts and partially normalizes the abundance of target cDNA in the subtracted population. The clones are ligated into a vector, plated, picked, and sequenced. These genes are then available for analysis and further investigation by means of follow-on projects.
Upon Completion of the Subtraction, the Customer will Receive:
With our flexible pricing, CD Genomics can begin the suppression subtractive hybridization procedure with tissue, total RNA, or poly(A)+ mRNA that you provide. Pricing is based on how much of the preparation is done by the customer, how much starting material is provided, and how many clones are sequenced.