CD Genomics-the genomics service company
Search
Search CD Genomics
CD Genomics
EXPLORE AREAS OF INTEREST
CD Genomics
Home / Company / Platform / HiSeq 4000 Sequencing

HiSeq 4000 Sequencing

Bookmark and Share

Generating up to 1.5 Tb reads per run, the HiSeq 4000 enables significantly higher throughputs, faster sequencing, and reduced per-base-costs. HiSeq4000 is more than three times faster than the HiSeq 2500 and yields about 65% more reads per lane. The progress in speed and output is enabled by two new technologies: patterned flow cells and kinetic exclusion amplification. In contrast to the random clustering employed in previous HiSeqs, the clusters are now generated in ordered nano-wells to allow for higher cluster densities and unambiguous cluster identification.

HiSeq 4000 Sequencing

Highlights

  • Dramatically Increased Data Output and Throughput
    Maximize efficiency through innovative patterned flow cell technology
  • Exceptional Data Quality
    Leverage proven, industry-leading Illumina sequencing by synthesis chemistry
  • Unsurpassed Capacity
    Sequence 12 genomes, 100 transcriptomes, or 96 exomes in fewer than 3.5 days
Performance Parameters of the HiSeq 4000 System
HiSeq 4000 System
No. of Flow Cells per Run 1 or 2
Data Yield - 2 × 150 bp 1300–1500 Gb
Data Yield - 2 × 75 bp 650–750 Gb
Data Yield - 1 × 50 bp 210–250 Gb
Quality Scores - 2 × 50 bp ≥ 85% bases above Q30
Quality Scores - 2 × 75 bp ≥ 80% bases above Q30
Quality Scores - 2 × 150 bp ≥ 75% bases above Q30
Daily Throughput > 400 Gb
Run Time < 1–3.5 days
Human Genomes per Run1 up to 12
Exomes per Run2 up to 96
Transcriptomes per Run3 up to 100

1: Assumes >30× coverage of a human genome.
2: Assumes 100× coverage with 80% on target using 2 × 75 bp reads.
3: Assumes 50 million reads per sample.

Quote Request
Name:
Phone:
Organization:
* Email:
* Services & Products of Interest:
Project Description:
* Verification Code:
Please input "genomics" as verification code.
Feedback

Accept problems, make progress together, work hard to succeed

READ MORE
Featured Publications

Keep abreast of the latest application status

READ MORE
CONTACT CD GENOMICS

45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-275-3058
Fax: 1-631-614-7828
Email: info@cd-genomics.com