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HLA Typing

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CD Genomics is providing accurate and reliable HLA genotyping service using next-generation sequencing (NGS) technology by generating unambiguous, phase-resolved HLA sequencing results in a single assay.

HLA Typing

HLAs (Human Leucocyte Antigens) are integral cell membrane glycoproteins, the molecules are encoded by genes located within a 3.6 Mb region on chromosome 6p21, which play key roles in immunity.

HLA genes are the most polymorphic gene family found in the human genome, with more than 10,000 different HLA alleles reported to date, thus the capacity to mount an immune response can be dramatically different between individuals within a cohort selected from a single population. HLA genes have been strongly implicated in transplant rejection, autoimmune disease, vaccine pharmacogenomics, cancer, infectious diseases, and mate selection.

HLA genes contain five to eight exons and range in length from 4–17 Kb. Two distinct classes of structurally similar HLA antigens have been well characterized and differ in their tissue distribution - class I HLA-A, -B, -C antigens are expressed ubiquitously on nucleated cells, whereas the class II HLA-DR, -DQ, -DP antigens are normally expressed on B cells, monocytes, macrophage, dendritic cells and on activated T cells. HLA genotyping is the identification of the HLA-class I and class II gene polymorphisms for an individual. Unambiguous HLA typing is important in transplant matching and disease association studies.

High-resolution HLA genotyping by NGS using various amplification and sequencing is uniquely able to address limitations of traditional Sanger sequencing assays in patients requiring a higher level of HLA allele matching, enabling robust, simple, and high-quality analysis of the key HLA genes. Multiplexing enables large numbers of loci to be sequenced simultaneously during a single experiment.

HLA Typing

We can offer the genotyping of 11 all HLA loci (HLA-A, B, C, DRB1, DRB3/4/5, DQA1, DQB1, DPA1, and DPB1) to deliver a detailed and comprehensive report. Typing can also be done at different levels of resolution. We offer a worldwide service, with our high throughput service to process hundreds of samples at a time. We can only accept genomic DNA. Our aim is to deliver a high confidence and responsive service to our clients at realistic cost.

Features and Advantages:

  • Comprehensive Assay: One assay provides high-resolution sequencing, replacing multiple assays with the most up-to-date genotyping technologies.
  • Unambiguous Results: Deeper sequencing with long inserts and paired-end reads enables phasing of all exons and introns for more accurate, high-resolution HLA typing.
  • Sample-to-Report Solution: Complete workflow includes sample preparation, sequencing, data analysis, and reporting.

The service is offered for R&D / non-diagnostic purposes only and results must not be utilized to inform patient management decisions.

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CONTACT CD GENOMICS

45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-275-3058
Fax: 1-631-614-7828
Email: info@cd-genomics.com