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45-1 Ramsey Road, Shirley,
NY 11967, USA
Tel: 1-631-372-1052
Fax: 1-631-614-7828
Email:

Europe
Tel: 44-207-048-3343

16s rDNA Sequencing


16s/18s rDNA Sequencing

CD Genomics offers our clients 16s, 18s rRNA and ITS Metagenomics Sequencing service by Illumina MiSeq Platform. This platform enables unparalleled insight of bacterial or fungi communities, enabling researchers to study a variety of microbiomes such as soil and intestinal flora. We offer sequencing and analysis of the V3 and V4 region or other regions of the 16S rRNA gene, 18s gene and ITS gene. Small number and large number of metagenomic samples can be sequenced and analyzed with fast turnaround time.


Amplicon Workflow:

Amplicon Workflow

User‐defined forward and reverse primers that are complementary upstream and downstream of the region of interest are designed with overhang adapters, and used to amplify templates from genomic DNA. A subsequent limited‐cycle amplification step is performed to add multiplexing indices and Illumina sequencing adapters. Libraries are normalized and pooled, and sequenced on the MiSeq system.

We offer a full package of this service, from DNA extraction, PCR amplification, Library Preparation, Sequencing and Data Analysis. Customers may submit environmental sample for Bacteria/Fungi gDNA extraction services or submit purified genomic DNA. All DNA samples are validated for purity and yield prior to processing.


Application

  •  • Identification of constituent microbial populations in both clinical and environmental samples
  •  • Identification/tracking of potentially infectious bacteria or sources of bacterial contamination

Bioinformatics Service

  •  • Supply of the raw data (file format FASTQ) by our secured FTP
  •  • Confirmation of microorganism's homogenic data using rRNA database (NCBI)
  •  • Clustering (table of the cluster distribution, diagrams)
  •  • Bioinformatics statistics (contig and coverage distribution)
  •  • Data may also be processed using the Illumina BaseSpace 16S analysis pipeline for fast and accurate analysis of metagenomic data.

Taxonomic summary results

Sunburst Classification Chart

This sunburst chart shows the relative abundance of the classification results within each taxonomic level.

Top 20 Classification Results by Taxonomic Level

This bar chart shows the relative abundance of the top 20 classification results within each taxonomic level.

Classification Rate by Taxonomic Level


Top Phylum Classification Results
Top Class Classification Results

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CONTACT CD GENOMICS
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-372-1052
Fax: 1-631-614-7828
Email:

Europe
Tel: 44-207-048-3343

SERVICES
Sequencing
Microarray
Genotyping
Aptamers
Custom Libraries
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